The genome nucleotide sequence of a contemporary wild strain of measles virus and its comparison with the classical Edmonston strain genome.
The genome nucleotide sequence of a contemporary wild strain of measles virus and its comparison with the classical Edmonston strain genome.
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麻疹病毒当代野生株的基因组核苷酸序列及其与经典埃德蒙斯顿株基因组的比较。
DOI：
10.1006/viro.1999.9643
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发表时间：
1999
期刊：
影响因子：
3.7
通讯作者：
Y. Nagai
中科院分区：
文献类型：
--
作者：
M. Takeda;T. Sakaguchi;Y. Li;F. Kobune;A. Kato;Y. Nagai
到目前为止，已报道的唯一完整的麻疹病毒(MeV)基因组核苷酸序列是埃德蒙斯顿(ED)毒株及其衍生物，这些毒株是几十年前分离出来的，在实验室条件下广泛传代，似乎是非致病性的。对许多其他菌株的部分测序已经确定了>；/=15种基因类型。最新的分离株，包括那些典型的致病株，属于不同于埃德蒙斯顿型的基因类型。因此，ED和相关毒株的序列可能不能代表当时或任何时候在人类群体中传播的病理性麻疹的序列。考虑到这些问题，以及如此多的研究是基于ED相关菌株的事实，我们已经对最近分离的致病菌株9301B的整个基因组进行了测序。新分离株与经典ED株之间有465个核苷酸差异(2.93%)和114个氨基酸差异(2.19%)。对开放阅读框中非同义和同义替换的计算，以及对每个基因非编码区和管外调控区的直接比较，清楚地揭示了允许和不允许改变的区域。值得注意的是，大量的非同义替换似乎允许P框架保持重叠C框架的高度序列保守性。然而，对于任何替代，其原因和结果在很大程度上尚不清楚，这表明这两个菌株之间存在着相当大的差距，无法填补。本文报道的序列可作为当代野生型MeV的参考。
The only complete genome nucleotide sequences of measles virus (MeV) reported to date have been for the Edmonston (Ed) strain and derivatives, which were isolated decades ago, passaged extensively under laboratory conditions, and appeared to be nonpathogenic. Partial sequencing of many other strains has identified >/=15 genotypes. Most recent isolates, including those typically pathogenic, belong to genotypes distinct from the Edmonston type. Therefore, the sequence of Ed and related strains may not be representative of those of pathological measles circulating at that or any time in human populations. Taking into account these issues as well as the fact that so many studies have been based upon Ed-related strains, we have sequenced the entire genome of a recently isolated pathogenic strain, 9301B. Between this recent isolate and the classical Ed strain, there were 465 nucleotide differences (2.93%) and 114 amino acid differences (2.19%). Computation of nonsynonymous and synonymous substitutions in open reading frames as well as direct comparisons of noncoding regions of each gene and extracistronic regulatory regions clearly revealed the regions where changes have been permissible and nonpermissible. Notably, considerable nonsynonymous substitutions appeared to be permissible for the P frame to maintain a high degree of sequence conservation for the overlapping C frame. However, the cause and the effect were largely unclear for any substitution, indicating that there is a considerable gap between the two strains that cannot be filled. The sequence reported here would be useful as a reference of contemporary wild-type MeV.
影响因子：
11.4
作者：
RADECKE, F;SPIELHOFER, P;BILLETER, MA
通讯作者：
BILLETER, MA