Antimalarial Properties of Aqueous Crude Extracts of Gynostemma pentaphyllum and Moringa oleifera Leaves in Combination with Artesunate in Plasmodium berghei-Infected Mice.
Antimalarial Properties of Aqueous Crude Extracts of Gynostemma pentaphyllum and Moringa oleifera Leaves in Combination with Artesunate in Plasmodium berghei-Infected Mice.
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DOI：
10.1155/2016/8031392
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发表时间：
2016
影响因子：
2.2
通讯作者：
Saiphet B
中科院分区：
文献类型：
--
作者：
Somsak V;Borkaew P;Klubsri C;Dondee K;Bootprom P;Saiphet B
由于对抗疟药物具有耐药性的疟疾寄生虫的出现和传播，迫切需要发现和开发新的、安全的和负担得起的抗疟药物。在这方面，药用植物提取物是优化抗疟作用和恢复标准抗疟药物功效的目标。本研究的目的是确定绞股蓝和辣木叶提取物与青蒿琥酯对伯氏疟原虫感染小鼠的抗疟作用。基于腹腔注射1 × 107个寄生虫红细胞和随后发生寄生虫血症，使用在ICR小鼠中连续传代维持的伯氏疟原虫ANKA。这些感染的小鼠被用来研究青蒿琥酯（6 mg/kg）与500、1，000和2，000 mg/kg G. pentaphyllum和M.油茶叶提取物进行4天抑制试验。结果发现，这些提取物具有显着的抗疟活性（P < 0.05），且呈剂量依赖性，对G的抑制率分别为45%、50%和55%。pentaphyllum leaf extract，35、40、50%;油茶叶提取物此外，青蒿琥酯与这些提取物组合呈现出更高的抗疟活性，与单独处理的提取物相比，对G的抑制百分比为78%、91%和96%。pentaphyllum leaf extract，M.油茶叶提取物结果表明，G. pentaphyllum或M.用低剂量青蒿琥酯可提高含青蒿琥酯的油茶叶提取物的抗疟活性。因此，这些结果证明了这些提取物和青蒿琥酯在抗疟草药中的组合。
Due to the emergence and spread of malaria parasite with resistance to antimalarial drugs, discovery and development of new, safe, and affordable antimalarial are urgently needed. In this respect, medicinal plant extracts are targets to optimize antimalarial actions and restore efficacy of standard antimalarial drugs. The present study was aimed at determining the antimalarial activities of Gynostemma pentaphyllum and Moringa oleifera leaf extracts in combination with artesunate against Plasmodium berghei-infected mice. P. berghei ANKA maintained by serial passage in ICR mice were used based on intraperitoneal injection of 1 × 107 parasitized erythrocytes and subsequent development of parasitemia. These infected mice were used to investigate the antimalarial activity of artesunate (6 mg/kg) in combination with 500, 1,000, and 2,000 mg/kg of G. pentaphyllum and M. oleifera leaf extracts using 4-day suppressive test. It was found that these extracts showed significant (P < 0.05) antimalarial activity in dose-dependent manner with percentage of suppression of 45, 50, and 55% for G. pentaphyllum leaf extract and 35, 40, and 50% for M. oleifera leaf extract. Additionally, artesunate combined with these extracts presented higher antimalarial activity, compared to extract treated alone with percentage of suppression of 78, 91, and 96% for G. pentaphyllum leaf extract and 73, 82, and 91% for M. oleifera leaf extract. The results indicated that combination treatment of G. pentaphyllum or M. oleifera leaf extracts with artesunate was able to increase the antimalarial activity by using low dose of artesunate. Hence, these results justified the combination of these extracts and artesunate in antimalarial herbal remedies.