Experimental Transmission of Mayaro Virus by Aedes aegypti
Experimental Transmission of Mayaro Virus by Aedes aegypti
复制标题
DOI：
10.4269/ajtmh.2011.11-0359
复制
发表时间：
2011-10-01
影响因子：
3.3
通讯作者：
Tesh, Robert B.
中科院分区：
文献类型：
--
作者：
Long, Kanya C.;Ziegler, Sarah A.;Tesh, Robert B.
马雅罗热的暴发与马雅罗病毒（MAYV）在南美洲的森林循环传播有关。为了评估一种常见城市蚊子传播MAYV的可能性，对来自秘鲁伊基托斯的埃及伊蚊进行了实验室媒介能力研究。伊蚊口腔感染。埃及伊蚊的范围为0%（0/31）至84%(31/37)，血粉病毒滴度在3.4 log（10）至7.3 log（10）斑块形成单位(PFU)/mL之间。70%（21/30）的受感染蚊子通过唾液采集和接触哺乳小鼠传播MAYV。通过实时聚合酶链反应测定发热人体内的病毒RNA量，范围为2.7至5.3 log(10) PFU当量/mL。伊蚊的口服敏感性。埃及伊蚊到MAYV病毒在病毒携带者身上的滴度可能限制启动城市循环的机会；然而，由Ae传播MAYV。埃及伊蚊显示出该物种的媒介能力，并提示城市传播的潜力。
Outbreaks of Mayaro fever have been associated with a sylvatic cycle of Mayaro virus (MAYV) transmission in South America. To evaluate the potential for a common urban mosquito to transmit MAYV, laboratory vector competence studies were performed with Aedes aegypti from Iquitos, Peru. Oral infection in Ae. aegypti ranged from 0% (0/31) to 84% (31/37), with blood meal virus titers between 3.4 log(10) and 7.3 log(10) plaque-forming units (PFU)/mL. Transmission of MAYV by 70% (21/30) of infected mosquitoes was shown by saliva collection and exposure to suckling mice. Amount of viral RNA in febrile humans, determined by real-time polymerase chain reaction, ranged from 2.7 to 5.3 log(10) PFU equivalents/mL. Oral susceptibility of Ae. aegypti to MAYV at titers encountered in viremic humans may limit opportunities to initiate an urban cycle; however, transmission of MAYV by Ae. aegypti shows the vector competence of this species and suggests potential for urban transmission.