Pulsed electric field treatment for bacteria reduction and its impact on hospital wastewater
Pulsed electric field treatment for bacteria reduction and its impact on hospital wastewater
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DOI：
10.1016/j.chemosphere.2008.11.066
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发表时间：
2009-04-01
期刊：
影响因子：
8.8
通讯作者：
Bluhm, Hansjoachim
中科院分区：
文献类型：
--
作者：
Gusbeth, Christian;Frey, Wolfgang;Bluhm, Hansjoachim
在过去的几年里，脉冲电场（PEF）方法进入了多个应用领域。一个有前景的应用是医院废水的净化，这些废水中含有大量的致病菌，并且越来越多的抗生素耐药细菌。在本研究中，悬浮在缓冲溶液或大学医院废水中的恶臭假单胞菌被用作参考菌株。为了证明存活细菌的后代是否对电场产生适应，对存活的PEF处理细菌进行了重新培养，并在系列实验中进行10次脉冲（100 kV cm(-1)和600 ns脉冲持续时间）的脉冲。这个过程重复了30代。失活率用 3.5 +/- 0.8 log 的集落形成单位计算，并在 30 个循环中保持恒定。对核糖体操纵子可变基因间隔区的研究表明，在连续 PEF 处理实验期间，基因组结构的这一高度可变部分没有明显变化。通过umu试验分析了PEF处理过的医院废水、缓冲溶液和自来水的致突变性。大多数医院废水样品在 PEF 处理之前就已经表现出相当大的遗传毒性，但 PEF 处理并没有增加这种遗传毒性，甚至对于超过 250 J mL(-1) 的更高处理能量也没有增加。经过 PEF 处理的缓冲溶液和自来水没有引起遗传毒性。这项研究表明，PEF 处理是一种可持续的非化学细菌净化方法，没有任何副作用。 (c) 2009 Elsevier Ltd. 保留所有权利。
During the last years the pulsed electric field (PEF) method entered several fields of application. A promising application is the decontamination of hospital wastewater effluents, which are loaded with pathogenic and increasingly with antibiotic-resistant bacteria. For this study, Pseudomonas putida suspended in buffer solution or wastewater from university hospital was used as reference strain. To prove whether the descendent of the survival bacteria develop an adaptation to electric field, surviving PEF treated bacteria were recultivated and pulsed in serial experiments with 10 pulses (100 kV cm(-1) and 600 ns pulse duration). This procedure was repeated for 30 generations. The inactivation rate was calculated with 3.5 +/- 0.8 log of colony forming units and remained constant over 30 cycles. Investigations of the variable intergenic spacer region of the ribosomal operon demonstrated no visible changes in this highly variable part of the genome structure during the serial PEF treatment experiments. The mutagenicity of PEF treated hospital wastewater, buffer solutions and tap water was analyzed by the umu-test. Most hospital wastewater samples exhibit a considerable genotoxicity already before PEF treatment, but this was not increased by the PEF treatment, not even for higher treatments energies over 250 J mL(-1). No genotoxicity was induced in buffer solutions and tap water by PEF treatment. This study supports, that PEF treatment is a sustainable non-chemical method for bacterial decontamination without any adverse effects. (c) 2009 Elsevier Ltd. All rights reserved.