Carbonyl 13C-detect solution-state protein NMR experiments to circumvent amide-solvent exchange broadening: Application to β2-microglobulin
Carbonyl 13C-detect solution-state protein NMR experiments to circumvent amide-solvent exchange broadening: Application to β2-microglobulin
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羰基 13C 检测溶液态蛋白质 NMR 实验以规避酰胺-溶剂交换展宽：在 β2-微球蛋白中的应用
DOI：
10.1016/j.bbapap.2020.140593
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Miyanoiri Yohei
中科院分区：
文献类型：
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作者：
Yoshimura Yuichi;So Masatomo;Miyanoiri Yohei
蛋白质核磁共振光谱中的15n - 1h异核单量子相关（HSQC）技术存在谱线展宽效应，如不稳定质子与溶剂的化学交换和构象动力学残基的交换展宽。在HSQC光谱中没有观察到β2-微球蛋白残基S88的酰胺共振，但可以通过13c检测实验获得，避免了酰胺-溶剂交换增宽的问题。S88共振在HSQC光谱中超出检测范围的谱线展宽不是由于构象交换，而是由于发生在~103s−1数阶上的溶剂交换。
The15N–1H heteronuclear single-quantum correlation (HSQC) technique in protein NMR spectroscopy suffers from line-broadening effects, such as chemical exchange of labile protons with solvent, and exchange broadening for residues undergoing conformational dynamics.The amide resonance of β2-microglobulin residue S88 is not observed in the HSQC spectrum but can be obtained through13C-detect experiments that circumvent the problem of amide-solvent exchange broadening.Line broadening of S88 resonance beyond detection in the HSQC spectrum is not attributed to conformational exchange but rather to solvent exchange occurring on the order of ~103s−1.