An approach to rapid protein crystallization using nanodroplets
An approach to rapid protein crystallization using nanodroplets
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DOI：
10.1107/s0021889802001474
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发表时间：
2002-04-01
影响因子：
6.1
通讯作者：
Stevens, RC
中科院分区：
文献类型：
--
作者：
Santarsiero, BD;Yegian, DT;Stevens, RC
据报道，一种方法可以将所需蛋白质的量减少两个数量级，并将蒸汽扩散蛋白质结晶所需的时间减少十倍。原型高通量自动化系统用于使用小至 20 nL 的液滴体积，从 480 种条件的筛选中生产各种蛋白质的衍射质量晶体。这种方法可以显着减少蛋白质结构测定的时间和成本，并允许更大、更有效的结晶参数空间筛选。用最少量的蛋白质快速生产衍射质量晶体的能力使得结构基因组学和基于结构的药物发现中的高通量工作成为可能。
An approach that enables up to a two order of magnitude reduction in the amount of protein required and a tenfold reduction in the amount of time required for vapor-diffusion protein crystallization is reported. A prototype high-throughput automated system was used for the production of diffraction-quality crystals for a variety of proteins from a screen of 480 conditions using drop volumes as small as 20 nL. This approach results in a significant reduction in the time and cost of protein structure determination, and allows for larger and more efficient screens of crystallization parameter space. The ability to produce diffraction-quality crystals rapidly with minimal quantities of protein enables high-throughput efforts in structural genomics and structure-based drug discovery.