Morphological and phylogenetic analysis of Skeletonema costatum-like diatoms (Bacillariophyta) from the China Sea
Morphological and phylogenetic analysis of Skeletonema costatum-like diatoms (Bacillariophyta) from the China Sea
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DOI：
10.1080/09670260601149784
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发表时间：
2007-05-01
影响因子：
2.4
通讯作者：
Fan, Xiao-Lei
中科院分区：
文献类型：
--
作者：
Chen, Guo-Fu;Wang, Guang-Ce;Fan, Xiao-Lei
对采自中国近海沿着不同海域的5种中肋骨条藻样硅藻进行了光镜和扫描电镜观察。这五个分离株在形态上不同，只有一个（SK-XM）被确定为严格意义上的中肋骨条线虫。其他四个菌株没有对应的形态学上的任何描述的螺旋藻属物种。为了探讨我们的分离物和以前描述的假单胞菌属物种之间的关系，通过PCR扩增获得部分大亚基核糖体DNA（D1-D2）序列，并与其他假单胞菌属物种的已发表序列进行系统发育分析。中肋骨条藻（SK-XM）与S. subsalsium和我们的另一个分离物（SK-DH）。后者有一个短的终端rimportula和1：1和1：2 fultoportula链接。第三个分离物（SK-HH）每个细胞含有几个叶绿体，有完整的fultoportulae连接兄弟阀，并与S.托品康另外两个分离株（SK-BH、SK-FQ）形成短链，并具有独特的fultoportulac，其基部斜向瓣面，与S. dohrnii。序列距离分析表明，S.中肋骨（SK-XM）和SK-DH之间以及SK-HH、SK-FQ和SK-BH之间。分离物之间的遗传相似性决定了它们的地理位置。
Five Skeletonenia costatum-like diatoms isolated from different areas along the coast of the China Sea were studied with light microscopy and scanning electron microscopy. The five isolates differed in morphology, only one (SK-XM) being identified as Skeletonema costatum sensu stricto. The other four isolates did not correspond morphologically to any described Skeletonema species. In order to explore the relationships between our isolates and previously described Skeletonenia species, partial large subunit ribosomal DNA (D1-D2) sequences were obtained by PCR amplification, and included in a phylogenetic analysis with published sequences of other Skeletonenia spp. Skeletonenia costatum sensu stricto (SK-XM) grouped with S. subsalsium and another of our isolates (SK-DH). The latter had a short terminal rimportula and both 1:1 and 1:2 fultoportula linkages. A third isolate (SK-HH) contained several chloroplasts per cell, with intact fultoportulae linking sibling valves, and grouped in the same clade as S. tropicum. The other two isolates (SK-BH, SK-FQ) formed short chains and had peculiar fultoportulac that were oblique to the valve face at their bases and grouped with S. dohrnii. Sequence distance analysis indicated higher similarity between S. costatum (SK-XM) and SK-DH and between SK-HH, SK-FQ and SK-BH. Genetic similarity between the isolates paralleled their geographical location.