Conservation of palindromic and mirror motifs within inverted terminal repeats of mariner-like elements
Conservation of palindromic and mirror motifs within inverted terminal repeats of mariner-like elements
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DOI：
10.1016/j.jmb.2005.05.006
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发表时间：
2005-08-05
影响因子：
5.6
通讯作者：
Augé-Gouillou, C
中科院分区：
文献类型：
--
作者：
Bigot, Y;Brillet, B;Augé-Gouillou, C
转座酶的niariner-like元件（MLEs）特异性地以二聚体的形式结合到编码它的转座子的反向末端重复序列上。因此，如前所述，通过用Mos 1和Himar 1转座酶获得的生化数据，可以识别位于反向末端序列（ITR）内的两个结合基序。在这里，我们通过分析来自45个MLE的5'和3' ITR序列的核酸性质，考虑到转座酶结合ITR的事实，使用其CRO结合结构域和迄今为止研究的CRO结合位点的一般特征，来定义参与MLE转座酶与其ITR结合的基序。我们的研究结果表明，在所有的MLE ITR，外半部是更好地保存比内半部。更有趣的是，他们让我们能够表征保守的回文和镜像图案特定于每个“MLE物种”。回文基序的存在与转座酶二聚体的结合相关，而镜像基序的性质被证明是每个ITR中有助于稳定转座酶-ITR相互作用的弯曲的原因。(c)2005爱思唯尔有限公司保留所有权利。
The transposase of the niariner-like elements (MLEs) specifically binds as a dimer to the inverted terminal repeat of the transposon that encodes it. Two binding-motifs located within the inverted terminal sequences (ITR) are therefore recognized, as previously indicated, by biochemical data obtained with the Mos1 and Himar1 transposases. Here, we define the motifs that are involved in the binding of a MLE transposase to its ITR by analyzing the nucleic acid properties of the 5' and 3' ITR sequences from 45 MLEs, taking into account the fact that the transposase binds to the ITR, using its CRO binding domains and the general characteristics of the cro binding sites so far investigated. Our findings show that in all the MLE ITRs, the outer half was better conserved than the inner half. More interestingly, they allowed us to characterize conserved palindromic and mirror motifs specific to each "MLE species". The presence of the palindromic motifs was correlated to the binding of the transposase dimer, whereas the properties of the mirror motifs were shown to be responsible for the bend in each ITR that helps to stabilize transposase-ITR interactions. (c) 2005 Elsevier Ltd. All rights reserved.