The expression of Wnt4 is regulated by estrogen via an estrogen receptor alpha-dependent pathway in rat pituitary growth hormone-producing cells.
The expression of Wnt4 is regulated by estrogen via an estrogen receptor alpha-dependent pathway in rat pituitary growth hormone-producing cells.
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Wnt4的表达通过雌激素通过大鼠垂体生长激素产生细胞中的雌激素受体α依赖性途径调节。
DOI：
10.1267/ahc.09033
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发表时间：
2009-12-29
影响因子：
2.4
通讯作者：
Osamura RY
中科院分区：
文献类型：
--
作者：
Miyakoshi T;Kajiya H;Miyajima K;Takei M;Tobita M;Takekoshi S;Osamura RY
Wnt信号在细胞生物学和发育的许多方面都很重要。在小鼠雌性生殖道中，Wnt4、Wnt5a和Wnt7a在发情周期中表现出差异表达，提示它们参与雌性生殖生理。虽然垂体是调节生殖的主要腺体，但Wnt信号在这里的分子机制尚不清楚。我们阐明了Wnt4在雌激素处理的去卵巢雌性大鼠垂体中的亚细胞分布。wnt4mrna的表达显著增加，尤其是在发情前期，与发情期和备孕期相比。Wnt4蛋白存在于几乎所有生长激素（GH）产生细胞的细胞质中，仅存在于少数促甲状腺激素β (TSHβ)产生细胞的细胞质中。在大鼠垂体瘤（MtT/S）细胞中，雌激素受体拮抗剂ICI 182780能完全抑制雌激素诱导的Wnt4 mRNA的表达。因此，大鼠垂体GH细胞合成Wnt4，这是通过雌激素受体依赖途径介导的雌激素诱导的。
Wnt signaling is important in many aspects of cell biology and development. In the mouse female reproductive tract, Wnt4, Wnt5a, and Wnt7a show differential expression during the estrus cycle, suggesting that they participate in female reproductive physiology. Although the pituitary is a major gland regulating reproduction, the molecular mechanism of Wnt signaling here is unclear. We elucidated the subcellular distribution of Wnt4 in the pituitary of estrogen-treated ovariectomized female rats. Expression of Wnt4 mRNA increased dramatically, particularly in proestrus compared with estrus and metestrus. Wnt4 protein was observed in the cytoplasm of almost all growth hormone (GH)-producing cells and in only a few thyroid-stimulating hormone β (TSHβ)-producing cells. In rat GH-producing pituitary tumor (MtT/S) cells, estrogen-induced expression of Wnt4 mRNA was completely inhibited by estrogen receptor antagonist ICI 182,780 in vitro. Thus, rat pituitary GH cells synthesize Wnt4 and this is induced by estrogen mediated via an estrogen receptor alpha-dependent pathway.