Detection of Opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes by a PCR-based method
Detection of Opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes by a PCR-based method
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DOI：
10.1017/s0031182002002573
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发表时间：
2003-01-01
期刊：
影响因子：
2.4
通讯作者：
Kitikoon, V
中科院分区：
文献类型：
--
作者：
Maleewong, W;Intapan, PM;Kitikoon, V
建立了一种检测实验感染的螺类和鲤科鱼类体内的猫尾后睾吸虫的PCR方法。该方法基于由pOV-A6特异性探针序列设计的引物，得到330个碱基对的产物。在引入单个尾蚴或囊蚴的宿主组织匀浆中完成检测。PCR可以检测到人工接种在蜗牛中的单个尾蚴或人工接种在鱼样品中的单个囊蚴。该方法对所有受感染的螺或鱼的阳性率为100%。该方法没有产生与其他双殖吸虫DNA，如Hapleliis taichui，Centrocestus spp.，马来棘口吸虫、片形吸虫、动物寄生虫、异盘并殖吸虫或单睾吸虫属。该检测方法在螺类和鱼类中间宿主的流行病学调查以及淡水鱼食源性寄生虫的调查中具有很大的应用潜力。
A PCR procedure for the detection of Opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes was developed. This procedure was based on primers designed from a pOV-A6 specific probe sequence giving a 330 base-pair product. The detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. PCR can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated in a fish sample. The method gave a 100% positivity rate for all infected snails or fishes. The method did not yield a 330 base-pair amplified product with other digenean fluke DNAs such as Haplorchis taichui, Centrocestus spp., Echinostoma malayanum, Fasciola gigantica, animal schistosomes, Paragonimus heterotremus or Haplorchoides spp. The assay has great potential for application in epidemiological surveys of both snail and fish intermediate hosts as well as for investigation of foodborne parasites in freshwater fishes.