Expression of aquaporin 1 and 5 in the developing mouse inner ear and audiovestibular assessment of an Aqp5 null mutant.
Expression of aquaporin 1 and 5 in the developing mouse inner ear and audiovestibular assessment of an Aqp5 null mutant.
复制标题
水通道蛋白 1 和 5 在发育中的小鼠内耳中的表达以及 Aqp5 无效突变体的听前庭评估。
DOI：
10.1007/s10162-002-3033-7
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Choo,Daniel
中科院分区：
文献类型：
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作者：
Merves,Michele;Krane,CarissaM;Dou,Hongwei;Greinwald,JohnH;Menon,AnilG;Choo,Daniel
为了研究水通道蛋白1和5（分别为AQP1和AQP5）在内耳发育和功能中的潜在作用，我们定义了它们在发育中的小鼠内耳中的时空表达模式，并研究了Aqp5功能丧失的形态学和生理学影响。标准原位杂交（ISH）和免疫组织化学（IHC）检测用于表达研究，常规形态学，行为学和生理学评估的听觉和平衡在Aqp5null突变小鼠。AQP1首先在胚胎第10.5天（E10.5）的耳囊中检测到，但最终定位于特定的内耳和膜迷路周围的结缔组织细胞的非感觉部分。AQP5显示特定的耳蜗表达，第一次检测到在E15.5在非感觉上皮，后来限制在螺旋突起附近的蜗管侧壁。AQP5的表达持续通过出生后的时期与出生后第7天（P7）和P14之间的血管纹的表达域的变化。Byin原位杂交和免疫组织化学技术，转录和蛋白质表达模式之间的细微差异，注意到两个AQP1和5。虽然AQP5在发育中的小鼠内耳中动态表达，但成年Aqp5敲除小鼠在测试时显示正常的听力和正常的内耳结构发育。这些结果表明，多余的或替代的机制，可能调节水的稳态在发展和成熟的内耳。
To examine the potential roles of aquaporins 1 and 5 (AQP1 and AQP5, respectively) in inner ear development and function, we defined their spatial and temporal expression patterns in the developing mouse inner ear and examined the morphologic and physiologic effects of loss ofAqp5function. Standardin situhybridization (ISH) and immunohistochemical (IHC) assays were used for expression studies with routine morphologic, behavioral, and physiologic assessments of hearing and balance inAqp5null mutant mice. AQP1 was first detected at embryonic day 10.5 (E10.5) in the otocyst but eventually localized to specific nonsensory portions of the inner ear and connective tissue cells surrounding the membranous labyrinth. AQP5 displayed specific cochlear expression, first detectable at E15.5 in the nonsensory epithelium and later restricted to the lateral wall of the cochlear duct near the spiral prominence. AQP5 expression continued through postnatal periods with a change of expression domain to the stria vascularis between postnatal day 7 (P7) and P14. Byin situhybridization and immunohistochemical techniques, subtle differences between transcript and protein expression patterns were noted for both AQP1 and 5. Although AQP5 is dynamically expressed in the developing mouse inner ear, adultAqp5knockout mice show normal hearing when tested and normal inner ear structural development. These results suggest redundant or alternative mechanisms that likely regulate water homeostasis in the developing and mature inner ear.