Developmental expression in the mouse nervous system of the p49(3F12) SAP kinase
Developmental expression in the mouse nervous system of the p49(3F12) SAP kinase
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DOI：
10.1016/0169-328x(95)00181-q
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Miller, CA
中科院分区：
文献类型：
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作者：
Martin, JH;Mohit, AA;Miller, CA
有丝分裂原活化蛋白（MAP）激酶是脯氨酸导向的丝氨酸/苏氨酸激酶，对多种细胞外信号做出反应。这些激酶的一个亚组，应激活化蛋白（SAP）激酶，响应细胞应激磷酸化c-jun。使用单克隆抗体（MAb）3F 12，我们已经克隆和部分表征p49（3F 12）激酶，大鼠SAP β激酶的小鼠同源物，并描述其在成年和发育中的小鼠的表达。与以前报道的MAP和SAP激酶不同，它主要在成年小鼠神经系统的神经元中表达为2.7 kb的转录本。一个2.4 kb的转录本也在睾丸中表达。在免疫细胞化学上，MAb 3F 12在成人脑神经元的细胞质中装饰了一个环绕细胞核的环状结构，在胚胎中装饰了明显的核周点。原位杂交首次揭示了在有丝分裂后神经元中的表达，在胚胎第11天。该mRNA也在Neuro-2A神经母细胞瘤细胞系中表达，并且不响应于分化剂而上调。这种激酶的神经元特异性表明存在神经元特有的信号转导级联。由于该氨基酸序列在人和小鼠中高度保守，后者可作为该激酶的调控和表达的模型。
Mitogen-activated protein (MAP) kinases are proline-directed, serine/threonine kinases that respond to a variety of extracellular signals. A subgroup of these kinases, stress-activated protein (SAP) kinases, phosphorylate c-jun in response to cellular stress. Using monoclonal antibody (MAb) 3F12, we have cloned and partially characterized p49(3F12) kinase, a mouse homologue of the rat SAP beta kinase and described its expression in the adult and developing mouse. Unlike previously reported MAP and SAP kinases, it is primarily expressed as a 2.7 kb transcript in neurons in the nervous system of the adult mouse. A 2.4 kb transcript is also expressed in the testis. Immunocytochemically, MAb 3F12 decorates a loop-like structure encircling the nucleus in the cytoplasm of neurons in the adult brain, and distinct perinuclear dots in the embryos. In situ hybridization first reveals expression in post-mitotic neurons, on embryonic day 11. The mRNA is also expressed in the Neuro-2A neuroblastoma cell line and is not upregulated in response to differentiating agents. The neuronal specificity of this kinase suggests the presence of a signal transduction cascade unique to neurons. As the amino acid sequence is highly conserved in the human and mouse, the latter may serve as a model for regulation and expression of this kinase.