3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering
3D-printed bioactive scaffolds from nanosilicates and PEOT/PBT for bone tissue engineering
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DOI：
10.1093/rb/rby024
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发表时间：
2019-02-01
影响因子：
6.7
通讯作者：
Gaharwar, Akhilesh K.
中科院分区：
文献类型：
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作者：
Carrow, James K.;Di Luca, Andrea;Gaharwar, Akhilesh K.
增材制造（AM）在设计用于再生医学的3D支架方面显示出前景。然而，许多用于增材制造的合成生物材料是生物惰性的。在这里，我们报道了由聚环氧对苯二甲酸乙酯(PEOT)/聚对苯二甲酸丁二酯（PBT） （PEOT/PBT）共聚物和2D纳米硅酸盐合成的生物活性纳米复合材料，用于制造用于骨组织工程的3D支架。PEOT/PBT已被证明在体内支持钙化和骨结合能力，而二维纳米硅酸盐在缺乏成骨诱导剂的情况下诱导人间充质干细胞（hMSCs）的成骨分化。研究了纳米硅酸盐对PEOT/PBT结构、力学和生物性能的影响。具体来说，在PEOT/PBT中添加纳米硅酸盐可以抑制共聚物的降解速率，从而提高纳米复合材料在生理条件下的稳定性。然而，纳米硅酸盐的加入并没有显著增加支架的机械刚度。体外实验证明，在PEOT/PBT中添加纳米硅酸盐可以提高AM纳米复合材料的生物活性。hMSCs在含有纳米硅酸盐的支架上容易增殖，导致骨相关蛋白的显著上调和矿化基质的产生。纳米硅酸盐与PEOT/PBT的协同作用可用于骨组织工程生物活性支架的设计。
Additive manufacturing (AM) has shown promise in designing 3D scaffold for regenerative medicine. However, many synthetic biomaterials used for AM are bioinert. Here, we report synthesis of bioactive nanocomposites from a poly(ethylene oxide terephthalate) (PEOT)/poly(butylene terephthalate) (PBT) (PEOT/PBT) copolymer and 2D nanosilicates for fabricating 3D scaffolds for bone tissue engineering. PEOT/PBT have been shown to support calcification and bone bonding ability in vivo, while 2D nanosilicates induce osteogenic differentiation of human mesenchymal stem cells (hMSCs) in absence of osteoinductive agents. The effect of nanosilicates addition to PEOT/PBT on structural, mechanical and biological properties is investigated. Specifically, the addition of nanosilicate to PEOT/PBT improves the stability of nanocomposites in physiological conditions, as nanosilicate suppressed the degradation rate of copolymer. However, no significant increase in the mechanical stiffness of scaffold due to the addition of nanosilicates is observed. The addition of nanosilicates to PEOT/PBT improves the bioactive properties of AM nanocomposites as demonstrated in vitro. hMSCs readily proliferated on the scaffolds containing nanosilicates and resulted in significant upregulation of osteo-related proteins and production of mineralized matrix. The synergistic ability of nanosilicates and PEOT/PBT can be utilized for designing bioactive scaffolds for bone tissue engineering.