Gene expression changes are age-dependent and lobe-specific in the brown Norway rat model of prostatic hyperplasia.
Gene expression changes are age-dependent and lobe-specific in the brown Norway rat model of prostatic hyperplasia.
复制标题
在前列腺增生的棕色挪威大鼠模型中，基因表达变化具有年龄依赖性和叶特异性。
DOI：
10.1002/pros.20935
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Brown,TerryR
中科院分区：
文献类型：
--
作者：
Bethel,CarliseR;Chaudhary,Jaideep;Anway,MatthewD;Brown,TerryR
BACKGROUNDBenign prostatic hyperplasia (BPH) is an age‐related enlargement of the prostate, characterized by increased proliferation of stromal and epithelial cells. Despite its prevalence, the etiology of BPH is unknown.METHODSThe Brown Norway rat is a model for age‐dependent, lobe‐specific hyperplasia of the prostate. Histological analyses of the dorsal and lateral lobes from aged rats reveal focal areas characterized by increased numbers of luminal epithelial cells, whereas the ventral lobe is unaffected. This study examined differential gene expression by lobe and age in the Brown Norway rat prostate. The objective was to identify genes with different levels of expression in the prostate lobes from 4‐month (young) and 24‐month (old) animals, and to subsequently link changes in gene expression to mechanisms of prostate aging.RESULTSThe number of age‐dependent differentially expressed genes was greatest in the dorsal compared to the ventral and lateral lobes. Minimal redundancy was observed among the differentially expressed genes in the three lobes. Age‐related changes in the expression levels of 14 candidate genes in the dorsal, lateral and ventral lobes were confirmed by quantitative RT‐PCR. Genes that exhibited age‐related differences in their expression were associated with proliferation, oxidative stress, and prostate cancer progression, including topoisomerase II alpha (Topo2a), aurora kinase B (Aurkb), stathmin 1 (Stmn1), and glutathione S‐transferase pi. Immunohistochemistry for Topo2a, Aurkb, and Stmn1 confirmed age‐related changes in protein localization in the lateral lobe of young and aged prostates.CONCLUSIONThese findings provide clues to the molecular events associated with aging in the prostate. Prostate 69: 838–850, 2009. © 2009 Wiley‐Liss, Inc.
