Effect of culture conditions on the chemical composition and biological activities of Agaricus brasiliensisS!Wasser et al. (Agaricomycetideae)
Effect of culture conditions on the chemical composition and biological activities of Agaricus brasiliensisS!Wasser et al. (Agaricomycetideae)
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DOI：
10.1615/intjmedmushr.v8.i4.40
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发表时间：
2006-01-01
影响因子：
1.2
通讯作者：
Ohno, Naohito
中科院分区：
文献类型：
--
作者：
Hashimoto, Satoshi;Akanuma, Akiko M.;Ohno, Naohito
许多保健食品中都含有巴西蘑菇。提供在不同国家从不同菌株培养并采用不同生产工艺的果体和菌丝体。在本研究中，我们计划比较使用同一菌株和相同生产工艺，在不同条件下，即室内室内（IC）和室外野外（OC）培养的巴西巴西木的化学特性和生物活性。通过组分糖分析、核磁共振和体内抗肿瘤活性评估，从IC和OC制备的多糖组分具有相似的结构特征和生物活性。而蛋白质组分在质量上存在差异，即通过二维聚丙烯酰胺凝胶电泳检测到特定的蛋白质斑点，酶活性也存在差异，尤其是漆酶、多酚氧化酶、过氧化物酶和β -葡聚糖酶的活性。这些事实有力地表明，药用蘑菇的培养条件是评价其药理活性的关键。
Agaricus brasiliensis is included in many kinds of health foods. Both the fruit body and mycelium, cultured in various countries from various strains and using various production processes, are supplied. In the present study, we planned to compare the chemical properties and biological activities of A. brasiliensis cultured under different conditions, i.e., indoors in a house (IC) and outside on a field (OC), using the same strain and the same production process. Polysaccharide fractions prepared from IC and OC had similar structural features and biological activity, as assessed by component sugar analysis, nuclear magnetic resonance, and antitumor activity in vivo. In contrast, the protein fractions were qualitatively different, i.e., specific protein spots were detected by two-dimensional polyacrylamide gel electrophoresis, and the enzyme activities, especially laccase, poly-phenoloxidase, peroxidase, and beta-glucanase activities, were different. These facts strongly suggested that the culture conditions of medicinal mushrooms are keys for their pharmacological activity assessment.