In situ hybridization histochemistry: a technique for the study of gene expression in single cells.
In situ hybridization histochemistry: a technique for the study of gene expression in single cells.
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原位杂交组织化学：一种研究单细胞基因表达的技术。
DOI：
10.1089/dna.1983.2.157
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Roberts,JL
中科院分区：
文献类型：
--
作者：
Gee,CE;Roberts,JL
以生长激素和前阿片黑素皮质素基因在大鼠垂体中的表达模式为例，详细介绍了原位杂交组织化学技术在组织切片中的应用。对影响杂交效率和分辨率的参数进行了优化，使该技术可以用于识别多肽合成的位置，并在单细胞水平上研究激素对基因表达的调节。
The patterns of growth hormone and proopiomelanocortin gene expression in the rat pituitary were used to detail the application of thein situhybridization histochemistry technique in tissue sections. Parameters affecting the efficiency and resolution of hybridization have been optimized so that this technique can be used to identify sites of peptide synthesis and to study hormonal regulation of gene expression at the single cell level.