13C direct detected NMR increases the detectability of residual dipolar couplings
13C direct detected NMR increases the detectability of residual dipolar couplings
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DOI：
10.1021/ja0645436
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发表时间：
2006-11-29
影响因子：
15
通讯作者：
Piccioli, Mario
中科院分区：
文献类型：
--
作者：
Balayssac, Stephane;Bertini, Ivano;Piccioli, Mario
13C直接检测正成为鉴定在1H检测实验中逃避检测的残基信号的越来越有效的方法。我们研制了1H部分再偶联实验的脉冲序列，用于测量1JH αCα和1JHN偶联，其分辨率与常规1H探测实验相同。在没有任何基于1H的实验的情况下获得了一组一致的骨架，并被证明是有效的蛋白质溶液结构测定。
13C direct detection is becoming an increasingly efficient approach to identify signals of residues that escape detection in1H detected experiments. Pulse sequences have been developed to obtain1H partially recoupled experiments for the measurement of the1JHαCαand1JHNcouplings with the same resolution available in conventional1H detected experiments. A consistent set of backbonerdcobtained without any1H-based experiment has been obtained and shown to be effective for protein solution structure determination.