Common ancestry of iron oxide- and iron-sulfide-based biomineralization in magnetotactic bacteria
Common ancestry of iron oxide- and iron-sulfide-based biomineralization in magnetotactic bacteria
复制标题
DOI：
10.1038/ismej.2011.35
复制
发表时间：
2011-10-01
期刊：
影响因子：
11
通讯作者：
Lins, Ulysses
中科院分区：
文献类型：
--
作者：
Abreu, Fernanda;Cantao, Mauricio E.;Lins, Ulysses
磁小体是由趋磁细菌产生的原核生物细胞器，由纳米级的磁铁矿（Fe 3 O 4）或/和硫铁矿（Fe 3S 4）磁性晶体组成，并被脂质双层膜包裹。在产磁铁矿的趋磁细菌中，存在于磁小体膜中的蛋白质调节磁铁矿晶体的生物矿化。在这些微生物中，编码磁小体膜蛋白的基因以及参与构建磁铁矿磁小体链的基因（mam和p21基因）被组织在一个基因组岛中。然而，部分是因为目前没有在纯培养的灰云母生产趋磁细菌，很少有人知道在这些生物体的灰云母生物矿化过程中，包括是否有类似的基因参与的过程。在这里，使用非培养的技术，我们现在表明，MAM基因参与生产磁铁矿磁小体也存在于生产辉长岩趋磁细菌。这一发现表明，磁铁矿和硼硅矿的生物成矿作用并不像以前认为的那样是独立演化的（即趋磁性是多系的）。相反，这里提出的结果是一致的一个模型中，生物矿化磁小体的能力和拥有的mam基因是由细菌从一个共同的祖先，即，趋磁性状是单系的收购。The ISME Journal（2011）5，1634-1640; doi：10.1038/ismej.2011.35; 2011年4月21日在线发表
Magnetosomes are prokaryotic organelles produced by magnetotactic bacteria that consist of nanometer-sized magnetite (Fe3O4) or/and greigite (Fe3S4) magnetic crystals enveloped by a lipid bilayer membrane. In magnetite-producing magnetotactic bacteria, proteins present in the magnetosome membrane modulate biomineralization of the magnetite crystal. In these microorganisms, genes that encode for magnetosome membrane proteins as well as genes involved in the construction of the magnetite magnetosome chain, the mam and mms genes, are organized within a genomic island. However, partially because there are presently no greigite-producing magnetotactic bacteria in pure culture, little is known regarding the greigite biomineralization process in these organisms including whether similar genes are involved in the process. Here using culture-independent techniques, we now show that mam genes involved in the production of magnetite magnetosomes are also present in greigite-producing magnetotactic bacteria. This finding suggest that the biomineralization of magnetite and greigite did not have evolve independently (that is, magnetotaxis is polyphyletic) as once suggested. Instead, results presented here are consistent with a model in which the ability to biomineralize magnetosomes and the possession of the mam genes was acquired by bacteria from a common ancestor, that is, the magnetotactic trait is monophyletic. The ISME Journal (2011) 5, 1634-1640; doi:10.1038/ismej.2011.35; published online 21 April 2011