Characterization of bioactive films loaded with melatonin and regulation of postharvest ROS scavenging and ascorbate-glutathione cycle in Agaricus bisporus
Characterization of bioactive films loaded with melatonin and regulation of postharvest ROS scavenging and ascorbate-glutathione cycle in Agaricus bisporus
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双孢蘑菇中负载褪黑激素的生物活性膜的表征以及采后ROS清除和抗坏血酸-谷胱甘肽循环的调节
DOI：
10.1016/j.postharvbio.2022.112107
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发表时间：
2022-09-15
影响因子：
7
通讯作者：
Wang, Jia
中科院分区：
文献类型：
--
作者：
Feng, Lei;Jiang, Xin;Wang, Jia
生物活性包装具有保护环境、保存产品等优点，具有良好的发展前景。褪黑激素是一种天然、无毒、无味的绿色防腐剂。在这项研究中，三种类型的生物活性膜开发利用褪黑激素浓度为30 mM，60 mM和90 mM。结果表明，机械，阻隔性和热性能的G/T/M-60膜是比较好的。此外，在将褪黑激素添加到G/T/M-60膜中之后，没有观察到对膜基质的损伤，并且褪黑激素分子被包裹在基质中。因此，我们选择了G/T/M-60膜作为双孢蘑菇褪黑激素的释放和包装膜。与对照组相比，G/T/M-60保持了白色蘑菇的白度和硬度，降低了呼吸速率、过氧化氢、超氧阴离子水平。它不仅能抑制多酚氧化酶（PPO）的活性，而且能保持较高的抗氧化酶活性。此外，G/T/M-60调节AsA-GSH循环系统，大大增加抗坏血酸和谷胱甘肽的含量，并为循环系统提供充足的底物，从而有效地去除过量的活性氧，从而减少对蘑菇膜脂的损伤。总之，G/T/M-60包装有效地保持了采后白色蘑菇的品质，延缓了其衰老，为蘑菇的保鲜和生物活性包装的探索提供了新的见解和技术指导。
Bioactive packaging has a good development prospect owing to its protecting environment and preserving products. Melatonin is a natural, non-toxic, and odorless green preservative. In this study, three types of biologically active films were developed utilizing melatonin concentrations of 30 mM, 60 mM, and 90 mM. The results showed that the mechanical, barrier and thermal properties of the G/T/M-60 film were comparatively good. Additionally, no damage to the film matrix was observed after adding melatonin to the G/T/M-60 film, and the melatonin molecules were wrapped in the matrix. Therefore, we selected the G/T/M-60 film for melatonin release and packaging of Agaricus bisporus. Compared with the control group, the G/T/M-60 maintained the whiteness and hardness of white mushrooms, and decreased the respiratory rate, hydrogen peroxide, superoxide anion levels. It not only inhibited the activity of polyphenol oxidase (PPO), but also maintained high activities of antioxidant enzymes. Further, the G/T/M-60 regulated the AsA-GSH circulatory system, greatly increased the ascorbic acid and glutathione content, and provided sufficient substrate for the circulatory system, which effectively removed excess reactive oxygen species, and thereby reduced the damage to membrane lipids in the mushrooms. In brief, the G/T/M-60 packaging effectively maintained the quality and delayed the senescence of postharvest white mushrooms, which provides new insights and technical guidance for the preservation of mushrooms and exploration of bioactive packaging.