Cell interaction with the extracellular matrices produced by endothelial cells and fibroblasts.
Cell interaction with the extracellular matrices produced by endothelial cells and fibroblasts.
复制标题
细胞与内皮细胞和成纤维细胞产生的细胞外基质的相互作用。
DOI：
10.1016/0014-4827(85)90469-0
复制
发表时间：
1985
影响因子：
3.7
通讯作者：
Vlodavsky,I
中科院分区：
文献类型：
--
作者：
Fridman,R;Alon,Y;Doljanski,F;Fuks,Z;Vlodavsky,I
比较了牛角膜内皮细胞和鸡胚成纤维细胞产生的细胞外基质（ECM）对细胞贴壁、增殖和分化的诱导作用。角膜内皮细胞外基质（cECM）诱导了一个相当的和快速的附着和扁平化的人尤文肉瘤和结肠癌细胞，分别利用纤连蛋白和层粘连蛋白作为粘附糖蛋白。相比之下，由成纤维细胞产生的ECM（fECM）容易支持尤文肉瘤细胞的附着和扁平化，但对癌细胞只有很小的影响。两种基质均刺激血管内皮细胞增殖，但fECM刺激程度较低。相比之下，紧密贴壁、非重叠和接触抑制的内皮细胞单层的形成仅由cECM决定。在fECM上培养的血管内皮细胞在彼此之上生长，并且甚至在汇合后期掺入[3H]胸苷。Neunte生长（睫状神经节细胞）和网络形成（成年大鼠少突胶质细胞）促进这两种类型的矩阵，但在一个更一致的方式与cECM。这可能是由于鸡胚成纤维细胞沉积到其ECM中的少量层粘连蛋白导致其有效诱导neunte生长以及有限程度的癌细胞附着和扁平化。因此，这表明，cECM和fECM之间的化学组成和超分子排列的差异不仅导致细胞的附着，扩散和增殖反应的差异，而且还导致其特征性形态外观和分化功能的表达。
The extracellular matrices (ECM) produced by cultured bovine corneal endothelial cells and chick embryo fibroblasts were compared for their induction of cell attachment, proliferation and differentiation. The corneal endothelial ECM (cECM) induced a comparable and rapid attachment and flattening of both human Ewing's sarcoma and colon carcinoma cells which utilize fibronectin and laminin as adhesive glycoproteins, respectively. In contrast, the ECM produced by fibroblasts (fECM) readily supported the attachment and flattening of Ewing's sarcoma cells but had only a small effect on the carcinoma cells. Vascular endothelial cells were stimulated to proliferate by both types of matrices, but to a lesser extent by the fECM. In contrast, the formation of a closely apposed, non-overlapping and contact-inhibited endothelial cell monolayer was only dictated by the cECM. Vascular endothelial cells cultured on fECM grew on top of each other and incorporated [3H]thymidine even late at confluency. Neunte outgrowth (ciliary ganglion cells) and network formation (adult rat oligodendrocytes) were promoted by both types of matrices but in a more consistent manner with the cECM. It is likely that the small amounts of laminin deposited by chick embryo fibroblasts into their ECM are responsible for its efficient induction of neunte outgrowth and for the limited degree of carcinoma cell attachment and flattening. It is thus demonstrated that differences in chemical composition and supramolecular arrangement between cECM and fECM result not only in differences in the attachment, spreading and proliferative responses of cells but also in the expression of their characteristic morphological appearance and differentiated functions.