Investigation on the effects of the atmospheric pressure plasma on wound healing in diabetic rats.
Investigation on the effects of the atmospheric pressure plasma on wound healing in diabetic rats.
复制标题
DOI：
10.1038/srep19144
复制
发表时间：
2016-02-23
影响因子：
4.6
通讯作者：
Chalangari KM
中科院分区：
文献类型：
--
作者：
Fathollah S;Mirpour S;Mansouri P;Dehpour AR;Ghoranneviss M;Rahimi N;Safaie Naraghi Z;Chalangari R;Chalangari KM
据估计，全世界15%的糖尿病患者患有糖尿病性溃疡。本研究的目的是提出一种非热大气等离子体治疗糖尿病伤口的新方法。等离子体由高压（8千伏）和高频（6千赫）电源产生的电离氦气组成。通过血管内注射链脲佐菌素诱导大鼠糖尿病。然后将血浆注入大鼠的人工静电创口10分钟。免疫组化法检测转化生长因子（TGF-β1）细胞因子水平。结果显示，糖尿病动物创面愈合率低于非糖尿病动物创面愈合率（P < 0.05）。血浆可提高非糖尿病大鼠创面愈合率（P < 0.05），且血浆处理后创面明显收缩（P < 0.05）。组织学分析显示表皮层形成，新生血管形成和细胞增殖。血浆处理也导致TGF-β1细胞因子从组织培养基中释放出来。本研究结果证实了血浆治疗对糖尿病大鼠创面愈合的作用。
It is estimated that 15 percent of individuals with diabetes mellitus suffer from diabetic ulcers worldwide. The aim of this study is to present a non-thermal atmospheric plasma treatment as a novel therapy for diabetic wounds. The plasma consists of ionized helium gas that is produced by a high-voltage (8 kV) and high-frequency (6 kHz) power supply. Diabetes was induced in rats via an intravascular injection of streptozotocin. The plasma was then introduced to artificial xerograph wounds in the rats for 10 minutes. Immunohistochemistry assays was performed to determine the level of transforming growth factor (TGF-β1) cytokine. The results showed a low healing rate in the diabetic wounds compared with the wound-healing rate in non-diabetic animals (P < 0.05). Moreover, the results noted that plasma enhanced the wound-healing rate in the non-diabetic rats (P < 0.05), and significant wound contraction occurred after the plasma treatment compared with untreated diabetic wounds (P < 0.05). Histological analyses revealed the formation of an epidermis layer, neovascularization and cell proliferation. The plasma treatment also resulted in the release of TGF-β1 cytokine from cells in the tissue medium. The findings of this study demonstrate the effect of plasma treatment for wound healing in diabetic rats.