Abnormal circulating amino acid profiles in multiple metabolic disorders
Abnormal circulating amino acid profiles in multiple metabolic disorders
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多种代谢性疾病中循环氨基酸谱异常
DOI：
10.1016/j.diabres.2017.07.023
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发表时间：
2017-10-01
影响因子：
5.1
通讯作者：
Feng, Rennan
中科院分区：
文献类型：
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作者：
Okekunle, Akinkunmi Paul;Li, Ying;Feng, Rennan
目的：探讨肥胖、2型糖尿病(T2D)和代谢综合征(METS)患者的循环氨基酸(AA)谱。方法：采用超高效液相色谱-串联四联质谱(UPLC-TQ-MS)技术，对200例按性别、年龄、体重指数匹配的健康、肥胖、2型糖尿病和METS患者的血清AA谱进行分析。采用Meta分析方法，对肥胖、T2D和METS患者血清AA水平进行Meta分析，共47篇病例对照研究(包括本研究)，探讨肥胖、T2D和Mets在AA水平上的差异。结果：与健康对照组相比，所有非健康受试者血清AA水平均有显著变化(P<；0.05)。谷氨酸(肥胖：18.62[11.64，25.61]mmol/L，P<；0.001)；谷氨酸(肥胖：18.62[11.64，25.61]mmol/L，P<；)；0.001，T2D：19.94[0.28，39.61]mmol/L，P<；0.05，蛋氨酸：12.45[3.98，20.91]mmol/L，P<；0.001)，Pro(肥胖：16.72[6.20，27.24]mmol/L，P<；0.001，T2D：20.72[15.82，25.61]mmol/L，P<；0.001，METS：29.95[25.18，34.71]mmol/L，P<；0.001)和异亮氨酸(肥胖：11.39[8.54，14.24]mmol/L，P<；0.001，T2D：7.37[1.5，13.22]mmol/L，P<；0.001，METS：10.40[4.9，15.89]mmol/L，P<；0.001)显著高于健康对照组。甘氨酸(肥胖：-30.99[-39.69，-22.29]mmol/L，P<；0.001，T2D：-30.37[-41.80，-18.94]mmol/L，P<；0.001；蛋氨酸：-35.24[-39.28，-31.21]mmol/L，P<；0.001)显著低于健康对照组。结论：在病例对照研究和荟萃分析中，肥胖与AA循环变化最多有关，其次是蛋氨酸和T2D。在所有代谢紊乱中，缬氨酸、异亮氨酸、谷氨酸和脯氨酸升高，而甘氨酸降低。(C)2017爱思唯尔B.V.保留所有权利。
Aim: To evaluate circulating amino acids (AA) profiles in obesity, type 2 diabetes (T2D) and metabolic syndrome (MetS).Methods: Serum AA were profiled among 200; healthy, obese, T2D and MetS subjects matched by sex, age and BMI using ultra- high performance liquid chromatography tandem quadruple mass spectrometry (UPLC- TQ- MS). A meta- analysis, including 47 case- control studies (including the current study) on serum AA in obesity, T2D and MetS searched through October 2016 was conducted to explore the AA differences in obesity, T2D and MetS.Results: In comparison with healthy controls, 14 AA (10 increased and 4 decreased) were significantly altered (P < 0.05) in all non- healthy subjects. Also, mean differences of valine (obese: 34.13 [27.70, 40.56] mmol/L, P < 0.001, T2D: 19.49 [3.31, 35.68] mmol/L, P < 0.05, MetS: 29.18 [16.04, 42.33] mmol/L, P < 0.001), glutamic acid (obese: 18.62 [11.64, 25.61] mmol/L, P < 0.001, T2D: 19.94 [0.28, 39.61] mmol/L, P < 0.05, MetS: 12.45 [3.98, 20.91] mmol/L, P < 0.001), proline (obese: 16.72 [6.20, 27.24] mmol/L, P < 0.001, T2D: 20.72 [15.82, 25.61] mmol/ L, P < 0.001, MetS: 29.95 [25.18, 34.71] mmol/L, P < 0.001) and isoleucine (obese: 11.39 [8.54, 14.24] mmol/L, P < 0.001, T2D: 7.37 [1.52, 13.22] mmol/L, P < 0.05, MetS: 10.40 [4.90, 15.89] mmol/L, P < 0.001) were significantly higher compared to healthy controls. Similarly, mean differences of glycine (obese: -30.99 [-39.69, -22.29] mmol/L, P < 0.001, T2D: -30.37 [-41.80, -18.94] mmol/L, P < 0.001 and MetS: -35.24 [-39.28, -31.21] mmol/L, P < 0.001) were significantly lower compared to healthy controls.Conclusion: In both the case- control study and meta- analysis, obesity was related to the most circulating AA changes, followed by MetS and T2D. Valine, isoleucine, glutamic acid and proline increased, while Glycine decreased in all metabolic disorders. (C) 2017 Elsevier B.V. All rights reserved.