Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor.
Continuous, real-time monitoring of cocaine in undiluted blood serum via a microfluidic, electrochemical aptamer-based sensor.
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DOI：
10.1021/ja806531z
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发表时间：
2009-04-01
影响因子：
15
通讯作者：
Soh, H. Tom.
中科院分区：
文献类型：
--
作者：
Swensen, James S.;Xiao, Yi;Ferguson, Brian S.;Lubin, Arica A.;Lai, Rebecca Y.;Heeger, Alan J.;Plaxco, Kevin W.;Soh, H. Tom.
能够直接在复杂的未处理的水性样品中连续、实时测量小分子分析物的生物传感器系统的开发一直是一个重大挑战，并且仅针对有限数量的目标实现了成功实施。为了解决这个问题，我们在这里报告的微流控电化学适体为基础的传感器（MECAS）芯片，其中我们整合了目标特异性的DNA适体，折叠，从而产生电化学信号，响应于分析物与微流控检测系统。作为一个模型，我们证明了连续，实时（约1分钟的时间分辨率）检测的小分子药物可卡因在接近生理，低微摩尔浓度直接在未稀释的，否则未经修改的血清。我们相信，我们将基于折叠的电化学传感器与小型化检测系统集成的方法可以为各种分子靶点的实时、即时检测奠定基础。
The development of a biosensor system capable of continuous, real-time measurement of small-molecule analytes directly in complex, unprocessed aqueous samples has been a significant challenge, and successful implementation has been achieved for only a limited number of targets. Towards a general solution to this problem, we report here the Microfluidic Electrochemical Aptamer-based Sensor (MECAS) chip wherein we integrate target-specific DNA aptamers that fold, and thus generate an electrochemical signal, in response to the analyte with a microfluidic detection system. As a model, we demonstrate the continuous, real-time (~1 minute time resolution) detection of the small molecule drug cocaine at near physiological, low micromolar concentrations directly in undiluted, otherwise unmodified blood serum. We believe our approach of integrating folding-based electrochemical sensors with miniaturized detection systems may lay the ground work for the real-time, point-of-care detection of a wide variety of molecular targets.
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影响因子：
16.6
作者：
Xiao, Y;Lubin, AA;Plaxco, KW
通讯作者：
Plaxco, KW
影响因子：
14.8
作者：
Xiao, Yi;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
7.4
作者：
Lubin, Arica A.;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
DOI：
10.1073/pnas.1633515100
发表时间：
2003-08-05
影响因子：
11.1
作者：
Fan, CH;Plaxco, KW;Heeger, AJ
通讯作者：
Heeger, AJ
影响因子：
2.9
作者：
Hamaguchi, N;Ellington, A;Stanton, M
通讯作者：
Stanton, M