Measurement of urinary lactoferrin as a marker of urinary tract infection
Measurement of urinary lactoferrin as a marker of urinary tract infection
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DOI：
10.1128/jcm.37.3.553-557.1999
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发表时间：
1999-03-01
影响因子：
9.4
通讯作者：
Nakanishi, H
中科院分区：
文献类型：
--
作者：
Arao, S;Matsuura, S;Nakanishi, H
评估了测量多形核白细胞释放的尿乳铁蛋白 (LF) 和设计用于测量尿 LF 的免疫层析测试条的有用性，以简单快速地诊断尿路感染 (UTI)。尿液标本采集自表面健康者和诊断患有 UTI 的患者。在初步研究中，通过酶联免疫吸附测定对 121 份正常标本和 88 份患者标本（其中 60 份患有 UTI）的 LF 浓度进行了定量。UTI 患者标本中的 LF 浓度为 3,300.0 +/- 646.3 ng/ml（平均值+/-标准误差），而健康人和无尿路感染患者的标本中分别为 30.4 +/- 2.7 ng/ml 和 60.3 +/- 14.9 ng/ml。根据这些结果，选择 200 ng/ml LF 浓度作为阴性的截止值。使用新设计的免疫层析（IC）试纸重新检查每个尿液样本，以计算功效指数。根据截断值计算出，与尿液标本镜检结果相比，IC试验的敏感性、特异性、阳性和阴性预测值分别为93.3%、89.3%、86.2%和94.9%。计算出UTI的相应指数为95.0、92.9、89.7和96.6%，测试在10分钟内完成。这些结果表明，使用 IC 试纸测量尿液 LF 为简单、快速诊断 UTI 提供了有用的工具。
The usefulness of the measurement of urinary lactoferrin (LF) released from polymorphonuclear leukocytes and of an immunochromatography test strip devised for measuring urinary LF for the simple and rapid diagnosis of urinary tract infections (UTI) was evaluated. Urine specimens were collected from apparently healthy persons and patients diagnosed as suffering from UTI, In the preliminary study, the LF concentrations in 121 normal specimens and 88 specimens from patients (60 with UTI) were quantified by an enzyme-linked immunosorbent assay, The LF concentration was 3,300.0 +/- 646.3 ng/ml (average +/- standard error of the mean) in the specimens from UTI patients, whereas it was 30.4 +/- 2.7 ng/ml and 60.3 +/- 14.9 ng/ml in the specimens from healthy persons and the patients without UTI, respectively. Based on these results, a 200-ng/ml LF concentration was chosen as the cutoff value for negativity. Each urine specimen was reexamined with the newly devised immunochromatography (IC) test strip to calculate the indices of efficacy. Based on the cutoff value, it was calculated that the sensitivity, specificity, and positive and negative predictive values of the IC test were 93.3, 89.3, 86.2, and 94.9%, respectively, compared with the results of the microscopic examination of the urine specimens for the presence of leukocytes. The respective indices for UTI were calculated as 95.0, 92.9, 89.7, and 96.6%, The tests were completed,within 10 min. These results indicated that urine LF measurement with the IC test strip provides a useful tool for the simple and rapid diagnosis of UTI.