Rhamnolipid influences biosorption and biodegradation of phenanthrene by phenanthrene-degrading strain Pseudomonas sp Ph6
Rhamnolipid influences biosorption and biodegradation of phenanthrene by phenanthrene-degrading strain Pseudomonas sp Ph6
复制标题
鼠李糖脂影响菲降解菌株假单胞菌 Ph6 对菲的生物吸附和生物降解
DOI：
10.1016/j.envpol.2018.04.125
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发表时间：
2018-09-01
影响因子：
8.9
通讯作者：
Ling, Wanting
中科院分区：
文献类型：
--
作者：
Ma, Zhao;Liu, Juan;Ling, Wanting
鉴于合成表面活性剂存在亚致死风险，鼠李糖脂是一类有前景的生物表面活性剂，有可能提高多环芳烃（PAHs）的生物可利用性，为合成表面活性剂提供一种良好的替代品。然而，以往很少有研究综合探讨鼠李糖脂影响多环芳烃生物吸附和生物降解背后的行为和机制。据我们所知，这是首次报道鼠李糖脂诱导的菲（PHE）降解菌株假单胞菌属Ph6中细菌胞膜调控的菲生物吸附和生物降解之间的联系。鼠李糖脂（0 - 400 mg/L）能够改变细胞表面的ζ电位、细胞表面疏水性（CSH）、细胞超微结构和官能团，进而改变Ph6对菲的生物吸附和生物降解。更多的菲吸附在细胞胞膜上会导致更多的菲扩散到细胞质中，从而有利于Ph6对菲的细胞内生物降解。鼠李糖脂（200 mg/L）阻碍了菲的生物吸附和生物降解。鼠李糖脂作为一种生物表面活性剂，可成功用作添加剂以改善环境中多环芳烃的微生物生物降解。（C）2018爱思唯尔有限公司。保留所有权利。
Given the sub-lethal risks of synthetic surfactants, rhamnolipid is a promising class of biosurfactants with the potential to promote the bioavailability of polycyclic aromatic hydrocarbons (PAHs), to provide a favorable substitute for synthetic surfactants. However, few previous studies have integrated the behavior and mechanism behind rhamnolipid-influenced PAH biosorption and biodegradation. This is, to our knowledge, the first report of a bacterial envelope regulated link between phenanthrene (PHE) biosorption and biodegradation by rhamnolipid-induced PHE-degrading strain Pseudomonas sp. Ph6. Rhamnolipid (0-400 mg L-1) can change the cell-surface zeta potential, cell surface hydrophobicity (CSH), cell ultra-microstructure and functional groups, and then alter PHE biosorption and biodegradation of Ph6. Greater amounts of PHE sorbed on cell envelopes results in more PHE diffusing into cytochylema, thus favoring PHE intracellular biodegradation of Ph6. Rhamnolipid (200 mg L-1) hindered PHE biosorption and biodegradation. Rhamnolipid, as a biosurfactant, can be successfully utilized as an additive to improve the microbial biodegradation of PAHs in the environments. (C) 2018 Elsevier Ltd. All rights reserved.