Tissue-specific repression of starvation and stress responses of the Neurospora crassa con-10 gene is mediated by RCO1.
Tissue-specific repression of starvation and stress responses of the Neurospora crassa con-10 gene is mediated by RCO1.
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DOI：
10.1006/fgbi.1998.1044
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发表时间：
1998-04
期刊：
影响因子：
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通讯作者：
K. Lee;D. Ebbole
中科院分区：
文献类型：
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作者：
K. Lee;D. Ebbole
粗神经孢子虫con-10基因在菌丝体中表达较弱，但在大孢子萌发过程中被诱导约1000倍。需要对调控con-10表达的启动子元件和反式作用因子进行研究，以获得对发育调控的详细了解。rco-1突变体显示con-10的基础表达水平提高了10倍。与野生型相比，rco-1突变体的菌丝体中con-10的表达在碳或氮饥饿和热休克下迅速诱导到高水平。尽管con-10在分生后期被诱导发育，但con-10在野生型菌丝体暴露于空气后不久就被热休克诱导。这些发现支持了RCO1是con-10的细胞类型特异性抑制因子的观点。我们认为RCO1的失活允许发育中的分生孢子调节con-10诱导的时间以应对胁迫。
The Neurospora crassa con-10 gene is weakly expressed in mycelia but is induced approximately 1000-fold during macroconidiation. Studies of the promoter elements and trans-acting factors that regulate con-10 expression are needed to gain a detailed understanding of developmental regulation. The rco-1 mutant displays a 10-fold elevated basal level of expression of con-10. In contrast to the wild type, con-10 expression in mycelia of the rco-1 mutant was rapidly induced to high levels by starvation for carbon or nitrogen and by heat shock. Although con-10 is developmentally induced late in conidiation, con-10 was inducible by heat shock shortly after exposure of the wild-type mycelium to air. These findings support the view that RCO1 is a cell type-specific repressor of con-10. We propose that inactivation of RCO1 allows developing conidiophores to adjust the timing of con-10 induction in response to stress.