Human cytochrome P-450 4 mRNA and gene: part of a multigene family that contains Alu sequences in its mRNA.
Human cytochrome P-450 4 mRNA and gene: part of a multigene family that contains Alu sequences in its mRNA.
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人细胞色素 P-450 4 mRNA 和基因：其 mRNA 中含有 Alu 序列的多基因家族的一部分。
DOI：
10.1073/pnas.83.18.6731
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发表时间：
1986
影响因子：
11.1
通讯作者：
Tukey,RH
中科院分区：
文献类型：
--
作者：
Quattrochi,LC;Pendurthi,UR;Okino,ST;Potenza,C;Tukey,RH
对几个重叠的lambda gt11基因克隆进行了测序，发现它们编码全长的人细胞色素P-450 4。此外，还从一个编码全长人P-450 4基因的lambda EMBL-3人基因组克隆中鉴定了外显子和内含子的结构和位置。人P-4504mRNA的侧翼有62个碱基对的5‘和1508个碱基对的3’非编码序列，有1548个碱基，编码一个516个氨基酸的蛋白质(Mr为58,376)。人P-450 4的氨基酸序列与小鼠和大鼠的同源性分别为69%和70%，与兔P-450 4的同源性为75%，与人的P-450的同源性为68%。该基因全长7.6kb，编码3118个核苷酸的外显子序列，由6个内含子分隔成7个外显子。外显子7由1802个核苷酸组成，包含三个串联排列的反转/互补Alu序列。人P-4504基因与人P1-450及相关基因在大鼠和小鼠中的基因组DNA序列比较，以及每个外显子-内含子连接处的氨基酸残基和三联体密码子的鉴定表明，人P-4504基因中每个内含子的位置在该基因家族中是保守的。虽然内含子在相关基因家族中的长度和同源性可能不是保守的，但内含子序列的位置可能是鉴定相关P-450基因的重要决定因素。
Several overlapping lambda gt11 cDNA clones have been sequenced and shown to encode for the full-length human cytochrome P-450 4. The structure and location of the exons and flanking intron regions were also identified from a lambda EMBL-3 human genomic clone that encodes the full-length human P-450 4 gene. The human P-450 4 mRNA is flanked by 62 base pairs of 5'- and 1508 base pairs of 3'-noncoding sequence, with 1548 bases that encode a protein of 516 amino acids (Mr, 58,376). The predicted amino acid sequence of human P-450 4 is 69% and 70% homologous to its equivalent in mouse and rat, respectively, 75% homologous to rabbit P-450 4, and 68% homologous to human P1-450. The 7.6-kilobase gene encodes 3118 nucleotides of exon sequence that is separated by six introns into seven exons. Exon 7, which is 1802 nucleotides, contains three inverse/complement Alu sequences that are organized in tandem. Comparison of the genomic DNA sequence of the human P-450 4 gene with the human P1-450 and related genes in rat and mouse and the identification of the amino acid residues and triplet codon at each exon-intron junction show that the location of each intron in the human P-450 4 gene is conserved within this gene family. Although the length and homology of the introns within a related gene family may not be conserved, the location of intronic sequences may be an important determinant in the identification of related P-450 genes.