Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance.
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DOI：
10.1016/j.neuron.2015.02.022
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发表时间：
2015-03-04
期刊：
影响因子：
16.2
通讯作者：
Zeng, Hongkui
中科院分区：
文献类型：
--
作者：
Madisen, Linda;Garner, Aleena R.;Shimaoka, Daisuke;Chuong, Amy S.;Klapoetke, Nathan C.;Li, Lu;van der Bourg, Alexander;Niino, Yusuke;Egolf, Ladan;Monetti, Claudio;Gu, Hong;Mills, Maya;Cheng, Adrian;Tasic, Bosiljka;Thuc Nghi Nguyen;Sunkin, Susan M.;Benucci, Andrea;Nagy, Andras;Miyawaki, Atsushi;Helmchen, Fritjof;Empson, Ruth M.;Knoepfel, Thomas;Boyden, Edward S.;Reid, R. Clay;Carandini, Matteo;Zeng, Hongkui
研究脑回路的一种日益强大的方法依赖于针对特定细胞类型的基因编码传感器和效应器。然而，目前的方法在功能和特异性方面仍然有限。在这里，我们利用几种交叉策略来产生多个转基因小鼠系，表达高水平的具有高特异性的新遗传工具。我们使用Cre/Flp和Cre/Dre双重组酶系统开发了驱动和双报告小鼠系和病毒载体，并建立了一个新的、可重靶向的基因组位点TIGRE，这使得大量Cre/tTA依赖报告系能够表达荧光蛋白、遗传编码的钙、电压或谷氨酸指示剂以及光遗传效应物，这些水平都比以前高得多。在GCaMP6、YCX2.60、VSFP Butterfly 1.2和Jaws的示例小鼠系中显示了高功能。这些新的转基因细胞系极大地扩展了监测和操纵神经元活动的能力，并增加了特异性。
An increasingly powerful approach for studying brain circuits relies on targeting genetically encoded sensors and effectors to specific cell types. However, current approaches for this are still limited in functionality and specificity. Here we utilize several intersectional strategies to generate multiple transgenic mouse lines expressing high levels of novel genetic tools with high specificity. We developed driver and double reporter mouse lines and viral vectors using the Cre/Flp and Cre/Dre double recombinase systems, and established a new, retargetable genomic locus, TIGRE, which allowed the generation of a large set of Cre/tTA dependent reporter lines expressing fluorescent proteins, genetically encoded calcium, voltage, or glutamate indicators, and optogenetic effectors, all at substantially higher levels than before. High functionality was shown in example mouse lines for GCaMP6, YCX2.60, VSFP Butterfly 1.2, and Jaws. These novel transgenic lines greatly expand the ability to monitor and manipulate neuronal activities with increased specificity.
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影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
DOI：
10.1073/pnas.94.2.575
发表时间：
1997-01-21
影响因子：
11.1
作者：
Chung, JH;Bell, AC;Felsenfeld, G
通讯作者：
Felsenfeld, G
影响因子：
25
作者：
Haddad, Rafi;Lanjuin, Anne;Madisen, Linda;Zeng, Hongkui;Murthy, Venkatesh N.;Uchida, Naoshige
通讯作者：
Uchida, Naoshige
影响因子：
16.2
作者：
Arenkiel, Benjamin R.;Peca, Joao;Feng, Guoping
通讯作者：
Feng, Guoping
影响因子：
46.9
作者：
Buchholz, F;Angrand, PO;Stewart, AF
通讯作者：
Stewart, AF