Microchip-based enumeration of human white blood cells.
Microchip-based enumeration of human white blood cells.
复制标题
基于微芯片的人类白细胞计数。
DOI：
10.1007/978-1-59745-426-1_5
复制
发表时间：
2007
期刊：
影响因子：
--
通讯作者：
McDevitt,JohnT
中科院分区：
文献类型：
--
作者：
Floriano,PierreN;Acosta,Shelley;Christodoulides,Nicolaos;Weigum,Shannon;McDevitt,JohnT
流式细胞术的出现极大地改变了进行医学检测的方式。然而，流式细胞仪的成本、大尺寸和维护需求使其在资源匮乏的环境中稀缺，几乎只有发达国家的临床病理实验室才能获得。由于细胞计数是诊断、预后和治疗的基本和关键支持，因此有必要采用另一种细胞计数方法，这种方法可能具有成本效益、便携和适合在资源匮乏的环境中使用。我们在这里描述了一种在简单的微流控结构中进行细胞计数实验的方案。该方案描述了如何建立一个简单的微流控细胞，并通过捕获和用抗CD45抗体免疫标记WBC来进行总WBC计数。
The advent of flow cytometry has considerably changed the ways in which medical testing is conducted. However, the cost of flow cytometers, their large size, and their maintenance needs make them scarce in resource-poor settings and available almost only in clinical pathology laboratories in developed countries. Because cell enumeration is a basic and crucial support of diagnosis, prognosis, and treatment, an alternative cell-counting method that would potentially be cost-effective, portable, and suitable for use in resource-poor settings is warranted. We describe here a protocol for conducting cell-counting experiments in a simple microfluidic structure. This protocol describes how to build a simple microfluidic cell and perform a total white blood cell (WBC) count through capture and immunolabeling of the WBCs with an anti-CD45 antibody.