Analysis of biosensors by chemically specific optical techniques.: Chemiluminescence-imaging and infrared spectroscopic mapping ellipsometry
Analysis of biosensors by chemically specific optical techniques.: Chemiluminescence-imaging and infrared spectroscopic mapping ellipsometry
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DOI：
10.1007/s00216-006-0805-7
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发表时间：
2007-03-01
影响因子：
4.3
通讯作者：
Volkmer, R.
中科院分区：
文献类型：
--
作者：
Hinrichs, K.;Gensch, M.;Volkmer, R.
目前用于读出微阵列的标准方法是荧光和化学发光成像技术。这些方法需要用标记标记成分，通常只检测标记分子的浓度。一种能够定量分析成分和组分相互作用的无标签成像方法将是很大的优势。在这篇文章中，它首次显示了红外测绘椭偏法能够在肽溶液孵育前后对生物芯片进行无标记成像。测量结果证明，红外椭偏仪是一种横向分辨识别不同材料和确定生物芯片组成的灵敏工具。所需的横向分辨率是通过使用红外同步加速器光束线的辐射来实现的。
The standard methods currently used to read out microarrays are fluorescent and chemiluminesent imaging techniques. These methods require labeling of a component with a marker and, usually, only the concentration of the marker molecule is detected. A label-free imaging method that also enables quantitative spectroscopic analysis of the composition and component interaction would be of great advantage. In this article it is shown for the first time that IR mapping ellipsometry enables label-free imaging of a biochip before and after incubation with peptide solution. The measurements prove that IR ellipsometry is a sensitive tool for laterally resolved identification of the different materials and determination of the composition of a biochip. The lateral resolution required was achieved by using radiation from an infrared synchrotron beamline.