HTRA3 expression in non-pregnant rhesus monkey ovary and endometrium, and at the maternal-fetal interface during early pregnancy
HTRA3 expression in non-pregnant rhesus monkey ovary and endometrium, and at the maternal-fetal interface during early pregnancy
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DOI：
10.1186/1477-7827-6-22
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发表时间：
2008-06-18
影响因子：
4.4
通讯作者：
Nie, Guiying
中科院分区：
文献类型：
--
作者：
Bowden, Marissa A.;Li, Ying;Nie, Guiying
背景：HTRA3是最近发现的哺乳动物丝氨酸蛋白酶家族HTRA（高温需要因子a）的成员。在啮齿类动物和人类中，HTRA3通过选择性剪接转录成两种mRNA（长和短）。我们之前已经表明，HTRA3在成熟大鼠卵巢中表达，并可能参与卵泡发生和黄体生成。HTRA3在小鼠和人类胎盘发育过程中也上调。目前的研究调查了HTRA3是否也定位于灵长类动物的卵巢（恒河猴n = 7）。此外，我们还检测了未怀孕的恒河猴子宫内膜（n = 4）和妊娠早期母胎界面（n = 5），以进一步研究HTRA3在灵长类动物子宫内膜和胎盘中的表达。方法：采用半定量RT-PCR法检测恒河猴组织中HTRA3 mRNA水平。免疫组化检测HTRA3蛋白表达和定位。结果：在卵巢、主动脉、膀胱、小肠、骨骼肌、心脏和子宫中均检测到长、短形式的HTRA3 mRNA，但在肝脏和肾脏中未检测到。HTRA3蛋白免疫定位于恒河猴卵巢所有卵泡期的卵母细胞。与原始、初级和次级卵泡颗粒细胞相比，晚期次生卵泡壁粒细胞和丘粒细胞的蛋白表达显著增加。窦卵泡壁粒细胞和积云粒细胞的表达也明显增加。与黄体叶黄素细胞相比，颗粒-叶黄素细胞的染色强度更高（n = 3）。在未怀孕的猴子宫内膜中，腺上皮中检测到HTRA3。基底子宫内膜腺的染色强度高于功能子宫内膜腺。在妊娠早期，HTRA3蛋白在母体蜕细胞和腺体中均可见强染色。结论：我们认为HTRA3可能参与灵长类动物卵巢卵泡发生和黄体生成。此外，与先前在人类中的发现相似，HTRA3可能是灵长类动物胎盘的一个因素，并且可能对其很重要。
Background: HTRA3 is a recently identified member of the mammalian serine protease family HTRA (high temperature requirement factor A). In both the rodent and the human HTRA3 is transcribed into two mRNA species (long and short) through alternative splicing. We have previously shown that HTRA3 is expressed in the mature rat ovary and may be involved in folliculogenesis and luteinisation. HTRA3 is also upregulated during mouse and human placental development. The current study investigated whether HTRA3 is also localised in the primate ovary (rhesus monkey n = 7). In addition, we examined the nonpregnant rhesus monkey endometrium (n = 4) and maternal-fetal interface during early pregnancy (n = 5) to further investigate expression of HTRA3 in primate endometrium and placentation.Methods: HTRA3 mRNA levels in several rhesus monkey tissues was determined by semiquantitative RT-PCR. Protein expression and localisation of HTRA3 was determined by immunohistochemistry.Results: Long and short forms of HTRA3 mRNA were detected in the ovary, aorta, bladder, small intestine, skeletal muscle, heart and uterus but not the liver nor the kidney. HTRA3 protein was immunolocalised to the oocyte of all follicular stages in the rhesus monkey ovary. Protein expression in mural and cumulus granulosa cells of late secondary follicles increased significantly compared to granulosa cells of primordial, primary and secondary follicles. Mural and cumulus granulosa cells of antral follicles also showed a significant increase in expression. Staining intensity was higher in the granulosa-lutein cells compared to the theca-lutein cells of corpora lutea (n = 3). In the non-pregnant monkey endometrium, HTRA3 was detected in the glandular epithelium. The basalis endometrial glands showed higher staining intensity than functionalis endometrial glands. During early pregnancy, strong staining for HTRA3 protein was seen in both maternal decidual cells and glands.Conclusion: We propose that HTRA3 may be involved in folliculogenesis and luteinisation in the primate ovary. Furthermore, similar to previous findings in the human, HTRA3 is possibly a factor involved in and potentially important for primate placentation.