Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins
Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins
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DOI：
10.1016/j.chroma.2008.04.024
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发表时间：
2008-06-06
影响因子：
4.1
通讯作者：
Lu, Xiuyang
中科院分区：
文献类型：
--
作者：
Jia, Guangtao;Lu, Xiuyang
本文研究了5种大孔吸附树脂对积雪草中积雪草苷和羟基积雪草苷的富集和分离性能。比较了HPD-100、HPD-300、X-5、AB-8和D-101大孔吸附树脂对纯度为80%的三萜总皂苷的吸附和解吸性能。结果表明，HPD-100对积雪草苷和羟基积雪草苷的吸附量和解吸量均高于其它树脂，且吸附速度快。采用HPD 100树脂填充柱进行动态吸附和解吸试验，优化了积雪草苷和羟基积雪草苷的分离工艺。积雪草提取物经HPD-100树脂梯度洗脱后，羟基积雪草苷含量由3.9%提高到39.7%，回收率为70.4%，积雪草苷含量由2.0%提高到21.5%，回收率为72.0%。结果表明，HPD 100树脂对羟基积雪草苷和积雪苷具有良好的分离效果，该方法可为其他中草药原料中三萜皂苷的分离提供参考。(c)2008 Elsevier B. V.保留所有权利。
In present study, the performance and separation characteristics of five macroporous resins for the enrichment and purification of asiaticoside and madecassoside from Centella asiatica extracts have been evaluated. The adsorption and desorption properties of total triterpene saponins (80% purity) on macroporous resins including HPD100, HPD300, X-5, AB-8 and D101 have been compared. According to our results, HPD100 offered higher adsorption and desorption capacities and higher adsorption speed for asiaticoside and madecassoside than other resins. Column packed with HPD100 resin was used to perform dynamic adsorption and desorption tests to optimize the separation process of asiaticoside and madecassoside from C. asiatica extracts. After the treatment with gradient elution on HPD100 resin, the content of madecassoside in the product increased from 3.9 to 39.7%, and the recovery yield was 70.4%; for asiaticoside the content increased from 2.0 to 21.5%, and the recovery yield was 72.0%. The results showed that HPD100 resin revealed a good ability to separate madecassoside and asiaticoside, and the method can be referenced for the separation of other triterpene saponins from herbal raw materials. (c) 2008 Elsevier B.V. All rights reserved.