Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization
Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization
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DOI：
10.1007/s004380050384
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发表时间：
1997-03-18
期刊：
影响因子：
--
通讯作者：
Khush, GS
中科院分区：
文献类型：
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作者：
Aggarwal, RK;Brar, DS;Khush, GS
栽培稻所属的稻属有 24 个物种（2n = 24 或 48），代表 7 个基因组（AA、BE、CC、EE、FF、BBCC 和 CCDD）。其中五个物种的基因组构成尚不清楚。这五个物种已被分为两个物种复合体，即四倍体ridleyi复合体（O.ridleyi、O.longiglumis）和二倍体meyeriana复合体（O.capsulata、O.meyeriana、O.indandamanica）。为了评估这些物种的基因组结构与其他已知稻基因组在分子水平上的差异，我们使用了全基因组 DNA 杂交方法。 23 个稻种、6 个相关属、5 个外群类群（2 个单子叶植物、3 个双子叶植物）和 6 个源自稻与野生种杂交的 F(1) 和 BC(1) 的 79 个种质的总基因组 DNA（限制性消化后）分别与来自 12 个稻种的 P-32 标记的总基因组 DNA 杂交： O. meyeriana、O. brachyantha、O. punctata、O. officinalis、O. eichingeri、O. alta、O. latifolia、O. australiensis 和 O. sativa。代表 Ridleyi 和 meyeriana 复合体的标记基因组 DNA 与其各自物种的所有种质交叉杂交效果最好，与代表稻及相关属其他基因组的交叉杂交效果较差，与外类群的交叉杂交效果较差。一般来说，在允许检测 70-75% 同源序列的条件下，无论存在还是不存在作为竞争剂的水稻 DNA，以信号强度测量的杂交差异均大于 50 倍。相比之下，当代表其他稻基因组的总DNA用作探针时，O.ridleyi和O.meyeriana复合体的物种没有表现出任何显着的交叉杂交。
The genus Oryza to which cultivated rice belongs has 24 species (2n = 24 or 48), representing seven genomes (AA, BE, CC, EE, FF, BBCC and CCDD). The genomic constitution of five of these species is unknown. These five species have been grouped into two species complexes, the tetraploid ridleyi complex (O. ridleyi, O. longiglumis) and the diploid meyeriana complex (O. granulata, O. meyeriana, O. indandamanica). To evaluate the genomic structure of these species in terms of divergence at the molecular level vis-a-vis other known genomes of Oryza, we used the total genomic DNA hybridization approach. Total genomic DNA (after restriction digestion) of 79 accessions of 23 Oryza species, 6 related genera, 5 outgroup taxa (2 monocots, 3 dicots) and 6 F(1)s and BC(1)s derived from crosses of O. sativa with wild species were hybridized individually with P-32-labeled total genomic DNA from 12 Oryza species: O. ridleyi, O. longiglumis, O. granulata, O. meyeriana, O. brachyantha, O. punctata, O. officinalis, O. eichingeri, O. alta, O. latifolia, O. australiensis, and O. sativa. The labeled genomic DNAs representing the ridleyi and meyeriana complexes cross-hybridized best to all the accessions of their respective species, less to those representing other genomes of Oryza and related genera, and least to outgroup taxa. In general, the hybridization differential measured in terms of signal intensities was >50-fold under conditions that permit detection of 70-75% homologous sequences, both in the presence and in the absence of O. sativa DNA as competitor. In contrast, when total DNAs representing other Oryza genomes were used as probes, species of the O. ridleyi and O. meyeriana complexes did not show any significant cross-hybridization (