Efficient pyruvate production by a multi-vitamin auxotroph of Torulopsis glabrata:: key role and optimization of vitamin levels
Efficient pyruvate production by a multi-vitamin auxotroph of Torulopsis glabrata:: key role and optimization of vitamin levels
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DOI：
10.1007/s002530100598
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发表时间：
2001-06-01
影响因子：
5
通讯作者：
Rui, XS
中科院分区：
文献类型：
--
作者：
Li, Y;Chen, J;Rui, XS
筛选出一株能利用氯化铵作为唯一氮源生产丙酮酸的多维生素营养缺陷型光滑球拟酵母WSH-IP 303。为了优化丙酮酸产量和生产力，一个简单但有用的，正交设计方法，被用来研究硫胺素，烟酸，吡哆醇，生物素，核黄素之间的关系。硫胺素被证实是影响丙酮酸产量的最重要因素。当硫胺素的浓度为0.01 mg/l或0.015 mg/l时，通过增加烟酸浓度来改善葡萄糖消耗。当烟酸、硫胺素、吡哆醇、生物素和核黄素的浓度分别为8.0、0.015、0.4、0.04和0.1 mg/l时，在48 h的摇瓶培养中，丙酮酸的浓度和产量分别达到52 g/l和0.52 g/g。通过采用最佳维生素浓度的组合，在2.5-1发酵罐中进行分批培养，初始葡萄糖浓度为112 g/l; 56 h后丙酮酸浓度达到69 g/l（产量为0.62 g/g）。
A multi-vitamin auxotroph, Torulopsis glabrata strain WSH-IP303, which can use ammonium chloride as a sole nitrogen source for pyruvate production, was selected. To optimize pyruvate yield and productivity, a simple but useful, orthogonal design method, was used to investigate the relationship between thiamine, nicotinic acid, pyridoxine, biotin, and riboflavin. Thiamine was confirmed to be the most important factor affecting pyruvate production. When the concentration of thiamine was 0.01 mg/l or 0.015 mg/l, glucose consumption was improved by increasing the nicotinic acid concentration. When the concentrations of nicotinic acid, thiamine, pyridoxine, biotin, and riboflavin were 8.0, 0.015, 0.4, 0.04, and 0.1 mg/l, respectively, pyruvate concentration and yield reached 52 g/l and 0.52 g/g, respectively, in a 48-h flask culture. By employing a combination of the optimum vitamin concentrations, a batch culture was conducted in a 2.5-1 fermenter with an initial glucose concentration of 112 g/l; and the pyruvate concentration reached 69 g/l after 56 h (yielding 0.62 g/g).