PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites.
PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites.
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DOI：
10.1007/978-1-4939-3067-8_10
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hafner, Markus
中科院分区：
文献类型：
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作者：
Danan, Charles;Manickavel, Sudhir;Hafner, Markus
在转录后基因调控 (PTGR) 过程中，RNA 结合蛋白 (RBP) 与所有类别的 RNA 相互作用，以控制 RNA 的成熟、稳定性、运输和翻译。在这里，我们描述了光活化核糖核苷增强交联和免疫沉淀 (PAR-CLIP)，这是一种转录组规模的方法，用于以核苷酸水平分辨率识别靶 RNA 上的 RBP 结合位点。该方法很容易适用于任何直接接触 RNA 的蛋白质，包括预计以序列或结构依赖性方式结合在离散 RNA 识别元件 (RRE) 上的 RBP，以及那些被认为短暂结合的蛋白质，例如 RNA 聚合酶或解旋酶。
During post-transcriptional gene regulation (PTGR), RNA binding proteins (RBPs) interact with all classes of RNA to control RNA maturation, stability, transport, and translation. Here, we describe Photoactivatable-Ribonucleoside-Enhanced Cross linking and Immunoprecipitation (PAR-CLIP), a transcriptome-scale method for identifying RBP binding sites on target RNAs with nucleotide-level resolution. This method is readily applicable to any protein directly contacting RNA, including RBPs that are predicted to bind in a sequence- or structure-dependent manner at discrete RNA recognition elements (RREs), and those that are thought to bind transiently, such as RNA polymerases or helicases.