Structure and mechanism in membrane trafficking
Structure and mechanism in membrane trafficking
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DOI：
10.1016/j.ceb.2010.03.011
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发表时间：
2010-08-01
影响因子：
7.5
通讯作者：
Reinisch, Karin M.
中科院分区：
文献类型：
--
作者：
Hughson, Frederick M.;Reinisch, Karin M.
长期以来，细胞生物学家一直对了解调节蛋白质和脂类在细胞内隔间移动的机制感兴趣。这种运输很大程度上是由囊泡(或其他膜载体)完成的，这些囊泡从一个隔室发芽并与另一个隔室融合。鉴于大型蛋白质复合体在囊泡运输中发挥的关键作用，最近的许多进展依赖于X射线结晶学和电子显微镜的结合使用。在这里，我们讨论了蛋白质的综合结构研究，这些蛋白质的组装将膜形成囊泡和小管，然后转向所谓的连接因子，这些因子似乎协调了囊泡对接和融合。
Cell biologists have long been interested in understanding the machinery that mediates movement of proteins and lipids between intracellular compartments. Much of this traffic is accomplished by vesicles (or other membranous carriers) that bud from one compartment and fuse with another. Given the pivotal roles that large protein complexes play in vesicular trafficking, many recent advances have relied on the combined use of X-ray crystallography and electron microscopy. Here, we discuss integrated structural studies of proteins whose assembly shapes membranes into vesicles and tubules, before turning to the so-called tethering factors that appear to orchestrate vesicle docking and fusion.