MiR200c Targets IRS1 and Suppresses Prostate Cancer Cell Growth
MiR200c Targets IRS1 and Suppresses Prostate Cancer Cell Growth
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MiR200c 靶向 IRS1 并抑制前列腺癌细胞生长
DOI：
10.1002/pros.22968
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发表时间：
2015-06-01
期刊：
影响因子：
2.8
通讯作者：
Zhou, Qiao
中科院分区：
文献类型：
--
作者：
Su, Wenjing;Xu, Miao;Zhou, Qiao
BackgroundThe downregulation of the tumor suppressor miR200c plays important roles in many malignant tumors. This study aims to show that miR200c is a posttranscriptional regulator of insulin receptor substrate 1 (IRS1) and over-expression of miR200c suppresses prostate cancer cell growth.MethodsBioinformatics analysis was used to show potential post-translational regulation of IRS1 by miR200c. Dual reporter gene assays were chosen to test the binding of miR200c to the potential seed sequences in IRS1 3UTR. RT-PCR, Q-PCR and western blot were applied to determine the regulation effect of miR200c on IRS1. CCK8 assay, soft agar assay, trypan blue exclusion assay and flow cytometric analysis were used to measure the biological effects of miR200c on prostate cancer cell proliferation and apoptosis.ResultsThe 449-455nt, 3061-3067nt, and 3096-3102nt of the IRS1 3-UTR were identified as three potential seed sequences for miR200c. MiR200c directly binds to IRS1 through the seed sequences in IRS1 3-UTR. Artificial overexpression of miR200c significantly downregulated the mRNA and protein levels of IRS1, together with decreased cell proliferation and increased cell death of PC3 and DU145 cells.ConclusionsOur results suggest that miR200c plays crucial roles in prostate cancer by post-transcriptional regulation of IRS1. The mir200c/IRS1 pathway may be a potential therapeutic target to prevent prostate cancer cell growth. Prostate 75:855-862, 2015. (c) 2015 Wiley Periodicals, Inc.
