Expression of TRAIL and its receptors DR5 and DcR2 in orthodontic tooth movement
Expression of TRAIL and its receptors DR5 and DcR2 in orthodontic tooth movement
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DOI：
10.14670/hh-28.933
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发表时间：
2013-07-01
影响因子：
2
通讯作者：
Loreto, C.
中科院分区：
文献类型：
--
作者：
Cardile, V.;Musumeci, G.;Loreto, C.
背景TRAIL是一种跨膜蛋白，可诱导包括牙槽骨在内的各种组织的凋亡。其体外表达可通过几种方法激活，例如RANKL给药和细胞刮除。检测破骨细胞样细胞中TRAIL及其受体DR 5和DcR 2的表达，分析其对细胞寿命的影响，并探讨其在正畸牙齿移动中的作用。通过RANKL刺激24 h（T1）、72 h（T2）或5天（T3），从小鼠造血细胞系中分化出破骨细胞样细胞;然后刮取一些培养物。通过免疫细胞化学和Western blot分析对照和处理细胞中的TRAIL、DR 5和DcR 2的免疫染色。在T1和T3时，在免疫细胞化学和Western印迹上，在经处理的破骨细胞样细胞中发现了显著更大的TRAIL表达。TRAIL表达在T1和T3达到峰值，分别对应于DcR 2和DR 5的最大值。这些数据可能有助于更好地了解调节牙齿移动的机制，并提高正畸治疗的准确性。
Background. TRAIL is a transmembrane protein that induces apoptosis in various tissues including alveolar bone. Its in vitro expression can be activated by several methods, such as RANKL administration and cell scraping. Expression of TRAIL and its receptors DR5 and DcR2 was examined in osteoclast-like cells to analyze their effects on cell lifespan and to explore their role in orthodontic tooth movement.Materials and Methods. Osteoclast-like cells were differentiated from a mouse hematopoietic cell line by stimulation with RANKL for 24 h (T1), 72 h (T2) or 5 days (T3); some cultures were then scraped. Immunostaining for TRAIL, DR5 and DcR2 was evaluated by immunocytochemistry and Western blot analysis in control and treated cells.Results. Significantly greater TRAIL expression was found in treated osteoclast-like cells at T1 and T3 both on immunocytochemistry and Western blotting. TRAIL expression peaked at T1 and T3 in correspondence with DcR2 and DR5 maxima, respectively.Conclusions. These data may contribute to a better understanding of the mechanisms regulating tooth movement and to improve the accuracy of orthodontic treatments.