SGN nerve filaments develop synapses with IHCs earlier than with OHCs in C57BL/6 mouse inner ear
SGN nerve filaments develop synapses with IHCs earlier than with OHCs in C57BL/6 mouse inner ear
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DOI：
10.26355/eurrev_202011_23791
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Chi, F-L
中科院分区：
文献类型：
--
作者：
Han, Z.;Ding, J.;Chi, F-L
目的：探讨C57BL/6小鼠内耳发育过程中毛细胞与螺旋神经节神经元（SGN）之间的联系。材料与方法：收集C57BL/6小鼠内耳标本，从E15（胚胎第15天）到成年小鼠；采用免疫组织化学方法对标本冰冻切片进行观察。结果：耳蜗的发育顺序是从基转至顶转。 Morphological development of SGNs occurs mainly from E16 to P12 (postnatal day 12). Hair cells appear from E18 to P12, and inner hair cells (IHCs) develop earlier than outer hair cells (OHCs).毛细胞和 SGN 之间的连接在 E18-P1 期间开始发育，形态上类似于 P8-P12 期间的成熟突触，并在成年小鼠中完全成熟。结论：听觉带突触的起源发生在 E18 至 P1 期间。与 SGN 和毛细胞的发育同步，功能丝仍与毛细胞相连，而备用丝则与毛细胞表面断开。 Connections between SGN nerve filaments and IHCs occur earlier than those between SGN nerve filaments and OHCs.
OBJECTIVE: To explore the connections between hair cells and spiral ganglion neurons (SGNs) during the development of the C57BL/6 mouse inner ear.MATERIALS AND METHODS: The specimens of C57BL/6 mouse inner ear, from E15 (embryo day 15) to adult mouse, were collected; immunohistochemistry was employed to explore the frozen sections of specimens.RESULTS: The development of cochlea starts sequentially from the basal turn to the apex turn. Morphological development of SGNs occurs mainly from E16 to P12 (postnatal day 12). Hair cells appear from E18 to P12, and inner hair cells (IHCs) develop earlier than outer hair cells (OHCs). The connections between hair cells and SGNs begin to develop during E18-P1, morphologically resemble mature synapses during P8-P12, and completely mature in adult mice.CONCLUSIONS: The genesis of auditory ribbon synapse occurs from E18 to P1. Synchronized with the development of SGNs and hair cells, the functional filaments remain connected to hair cells, while the spare ones get disconnected from the surface of hair cells. Connections between SGN nerve filaments and IHCs occur earlier than those between SGN nerve filaments and OHCs.