Improvement of cordycepin production by an isolated Paecilomyces hepiali mutant from combinatorial mutation breeding and medium screening
Improvement of cordycepin production by an isolated Paecilomyces hepiali mutant from combinatorial mutation breeding and medium screening
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DOI：
10.1007/s00449-021-02611-w
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发表时间：
2021-07-15
影响因子：
3.8
通讯作者：
Zheng, Yu-Guo
中科院分区：
文献类型：
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作者：
Cai, Xue;Jin, Jie-Yi;Zheng, Yu-Guo
虫草素是冬虫夏草中发现的一种主要生物活性化合物，具有广泛的生物活性。本研究首先从高原土壤中分离出一种蝙蝠蛾拟青霉OR - 1菌株，用于虫草素的生物合成。随后，采用钴 - 60γ射线和紫外线照射等菌株诱变方法来提高虫草素的效价，获得了高产突变菌株蝙蝠蛾拟青霉ZJB18001，其虫草素含量达到0.61毫克/克（干细胞重），是野生菌株（0.26毫克/克（干细胞重））的2.3倍。此外，基于Box - Behnken设计和响应面法进行培养基筛选，在优化的培养基组成下，于25℃液体深层培养5天，虫草素产量提高至0.96毫克/克（干细胞重）。蝙蝠蛾拟青霉ZJB18001的虫草素高产、生长速度快以及遗传特性稳定等特点，在虫草素工业化生产的成本和时间方面具有优势。
Cordycepin is a major bioactive compound found in Cordyceps sinensis that exhibits a broad spectrum of biological activities. Here a Paecilomyces hepiali OR-1 strain was initially isolated from plateau soil for the bioproduction of cordycepin. Subsequently, strain modification including Co-60 gamma-ray and ultraviolet irradiation were employed to increase the cordycepin titer, resulted in a high-yield mutant strain P. hepiali ZJB18001 with the cordycepin content of 0.61 mg/g(DCW), showing a 2.3-fold to that from the wild strain (0.26 mg/g(DCW)). Furthermore, medium screening based on Box-Behnken design and the response surface methodology facilitated the enhancement of cordycepin yield to the value of 0.96 mg/g(DCW) at 25 degrees C for 5 days in submerged cultivation with an optimized medium composition. The high cordycepin yield, rapid growth rate and stable genetic characteristics of P. hepiali ZJB18001 are beneficial in terms of costs and time for the industrialization of cordycepin production.