Culture and differentiation of osteoblasts on coral scaffold from human bone marrow mesenchymal stem cells
Culture and differentiation of osteoblasts on coral scaffold from human bone marrow mesenchymal stem cells
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DOI：
10.1007/s10561-010-9208-2
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发表时间：
2011-11-01
影响因子：
1.5
通讯作者：
Strong, D. Michael
中科院分区：
文献类型：
--
作者：
Cong Toai Tran;Gargiulo, Ciro;Strong, D. Michael
在本文中，我们描述了一种方法，其目的是提供基本的信息，对使用天然珊瑚支架，以启动间充质干细胞成骨分化的移植目的的科学，生物力学基础。珊瑚等生物材料是一种骨传导材料，可用于人类源性干细胞的归巢，用于临床再生目的。在骨移植中，生物材料的使用可能是绕过两个主要关键障碍的解决方案，即自体移植物供体部位的短缺和同种异体移植手术的排斥风险。骨再生通常需要用于多种临床目的，例如，在美学重建和再生程序中。珊瑚移植物Porites lutea已被我们的团队用于临床应用十年，超过一千名患有不同骨病变的患者，包括椎管狭窄和下颌骨重建。人骨髓（human bone marrow，hBM）是间充质干细胞（mesenchymal stem cells，MSCs）的重要来源，可分化为成骨细胞、软骨细胞、脂肪细胞、肌细胞、心肌细胞和神经元等多种细胞。使用富含两种特异性生长因子FGF9和维生素D2的成骨培养基将从人骨髓分离的MSC诱导成成骨细胞。将部分培养的MSCs直接接种于珊瑚支架（Porites Lutea）上诱导分化为成骨细胞，部分培养于培养瓶中进行成骨细胞培养。这些数据支持这样的概念，即hBM是一种可靠的MSC来源，其可以容易地分化成成骨细胞并作为临床应用的最佳装置接种到珊瑚中。在本课题中，我们还讨论了间充质干细胞的生物学特性、其在临床移植中的潜在应用以及其在基因治疗中的前景。
In this paper we describe an approach that aims to provide fundamental information towards a scientific, biomechanical basis for the use of natural coral scaffolds to initiate mesenchymal stem cells into osteogenic differentiation for transplant purposes. Biomaterial, such as corals, is an osteoconductive material that can be used to home human derived stem cells for clinical regenerative purposes. In bone transplantation, the use of biomaterials may be a solution to bypass two main critical obstacles, the shortage of donor sites for autografts and the risk of rejection with allograft procedures. Bone regeneration is often needed for multiple clinical purposes for instance, in aesthetic reconstruction and regenerative procedures. Coral graft Porites lutea has been used by our team for a decade in clinical applications on over a thousand patients with different bone pathologies including spinal stenosis and mandibular reconstruction. It is well accepted that human bone marrow (hBM) is an exceptional source of mesenchymal stem cells (MSCs), which may differentiate into different cell phenotypes such as osteoblasts, chondrocytes, adipocytes, myocytes, cardiomyocytes and neurons. Isolated MSCs from human bone marrow were induced into osteoblasts using an osteogenic medium enriched with two specific growth factors, FGF9 and vitamin D2. Part of the cultured MSCs were directly transferred and seeded onto coral scaffolds (Porites Lutea) and induced to differentiate into osteoblasts and part were cultured in flasks for osteocell culture. The data support the concept that hBM is a reliable source of MSCs which may be easily differentiated into osteoblasts and seeded into coral as an optimal device for clinical application. Within this project we have also discussed the biological nature of MSCs, their potential application for clinical transplantation and the prospect of their use in gene therapy.