Formation of tubules and giant vesicles from large multilamellar vesicles.
Formation of tubules and giant vesicles from large multilamellar vesicles.
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从大的多层囊泡形成小管和巨型囊泡。
DOI：
10.1016/s0021-9797(03)00687-8
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发表时间：
2003
影响因子：
9.9
通讯作者：
D. Renard
中科院分区：
文献类型：
--
作者：
J. Douliez;L. Lavenant;D. Renard
本研究报告了对亚微米多层囊泡 (MLV) 的观察，该亚微米多层囊泡 (MLV) 是通过简单地冻融完全水合的磷脂分散体制备的，当限制在显微镜载玻片之间时，亚微米多层囊泡 (MLV) 转化为巨大的囊泡 (GV) 和小管 (TU)。盖玻片清洁和表面处理不会妨碍 GV 或 TU 的形成。然而，当制备小单层囊泡 (SUV) 时，或者当 MLV 不受限制而是在载玻片之间自由移动时，或者当磷脂处于凝胶相时，都没有观察到 GV 和 TU。总而言之，我们的结果表明，MLV 将发挥脂质储存库的作用，载玻片之间的液体流动会诱导外部双层的剥离，从而形成小管和巨大的单层囊泡。
This study reports an observation of submicrometer multilamellar vesicles (MLVs) prepared by simply freeze–thawing a phospholipid dispersion at full hydration that transformed into giant vesicles (GVs) and tubules (TUs) when confined between microscope glass slides. Cover slide cleaning and surface treatment did not hamper the formation of GVs or TUs. However, when small unilamellar vesicles (SUV) were prepared or when MLVs were not confined but rather freely moved between the glass slides or when the phospholipid was in its gel phase, neither GVs nor TUs were observed. Altogether, our results suggested that MLVs would play a role as a lipid reservoir and that the liquid flow between the glass slides induces the peeling of the external bilayers, yielding the formation of tubules and giant unilamellar vesicles.
DOI：
10.1073/pnas.93.21.11443
发表时间：
1996-10-15
影响因子：
11.1
作者：
Moscho, A;Orwar, O;Zare, RN
通讯作者：
Zare, RN