FoxP2 expression defines dorsolateral pontine neurons activated by sodium deprivation
FoxP2 expression defines dorsolateral pontine neurons activated by sodium deprivation
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DOI：
10.1016/j.brainres.2010.11.028
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发表时间：
2011-02-23
期刊：
影响因子：
2.9
通讯作者：
Loewy, Arthur D.
中科院分区：
文献类型：
--
作者：
Geerling, Joel C.;Stein, Matthew K.;Loewy, Arthur D.
在脑桥背外侧的两个特定的神经元组被饮食钠剥夺激活。这两组分别是蓝斑前核（pre-LC）和臂旁外核（PBel-inner）的内部分支。在每个部位，给老鼠喂食极低钠饮食超过一周后，神经元增加了一种活性诱导转录因子c-Fos的表达。在这里，我们证实了这一观察结果，并通过证明这两组神经元表达一个共同的标记基因，组成表达的转录因子叉头盒蛋白2 （FoxP2）来扩展它。也就是说，几乎所有c-Fos激活的神经元在这两个区域也表达FoxP2。FoxP2在这两组神经元中的表达表明它们是来自同一基本群体的发育相关亚群。与c-Fos不同，FoxP2的表达不依赖于钠剥夺，因此该标记物可能在未来lc前和PBel-inner的研究中有用。这些神经元投射到影响内脏、食欲和享乐功能的前脑回路中，其分子定义可能允许使用遗传工具对这些回路的功能作用进行直接实验探索。(c) 2010 Elsevier B.V.保留所有权利。
Two specific groups of neurons in the dorsolateral pons are activated by dietary sodium deprivation. These two groups are the pre-locus coeruleus (pre-LC) and the inner subdivision of the external lateral parabrachial nucleus (PBel-inner). In each site, after rats are fed an extremely low-sodium diet for over a week, neurons increase their expression of an activity-induced transcription factor, c-Fos. Here, we confirm this observation and extend it by demonstrating that these two groups of neurons express a common marker gene, the constitutively-expressed transcription factor Forkhead box protein 2 (FoxP2). That is, virtually all of the c-Fos activated neurons in both regions also express FoxP2. The expression of FoxP2 by both these groups of neurons suggests that they are developmentally-related subsets derived from the same basic population. Given that FoxP2, unlike c-Fos, is expressed independent of sodium deprivation, this marker may be useful in future studies of the pre-LC and PBel-inner. The molecular definition of these neurons, which project to circuits in the forebrain that influence visceral, appetitive, and hedonic functions, may allow direct experimental exploration of the functional role of these circuits using genetic tools. (c) 2010 Elsevier B.V. All rights reserved.