Enhancement of whole body glucose uptake during and after human skeletal muscle low-frequency electrical stimulation
Enhancement of whole body glucose uptake during and after human skeletal muscle low-frequency electrical stimulation
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DOI：
10.1152/japplphysiol.00486.2002
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发表时间：
2003-06-01
影响因子：
3.3
通讯作者：
Nakao, K
中科院分区：
文献类型：
--
作者：
Hamada, T;Sasaki, H;Nakao, K
有相当多的证据表明，电刺激(ES)激活了啮齿动物骨骼肌的葡萄糖摄取。然而，目前尚不清楚ES是否能在人类中导致类似的代谢增强。我们通过放置在股四头肌运动点上的表面电极使用低频电刺激。在仰卧位的男性受试者中，以0.2ms的20赫兹双相正方形脉冲和1-S开关占空比的刺激模式获得了最高的摄氧量。在20分钟的刺激期间，摄氧量增加了大约两倍，并在刺激后立即恢复到基线水平。呼吸交换率和血乳酸浓度的同时升高表明ES期间无氧糖原的分解和利用。正糖钳夹期间，由葡萄糖处理率决定的全身葡萄糖摄取量显著增加2.5毫克。Kg(-1).min(-1)，且持续升高3-4 mg。Kg(-1)。Min(-1)，在刺激停止后至少90分钟。因此，ES对全身葡萄糖摄取的刺激作用不仅在刺激期间持续存在，而且在刺激后也持续存在。低频ES可能成为激活人类能量和葡萄糖代谢的一种有用的治疗方法。
There is considerable evidence to suggest that electrical stimulation (ES) activates glucose uptake in rodent skeletal muscle. It is, however, unknown whether ES can lead to similar metabolic enhancement in humans. We employed low-frequency ES through surface electrodes placed over motor points of quadriceps femoris muscles. In male subjects lying in the supine position, the highest oxygen uptake was obtained by a stimulation pattern with 0.2-ms biphasic square pulses at 20 Hz and a 1-s on-off duty cycle. Oxygen uptake was increased by approximately twofold throughout the 20-min stimulation period and returned to baseline immediately after stimulation. Concurrent elevation of the respiratory exchange ratio and blood lactate concentration indicated anaerobic glycogen breakdown and utilization during ES. Whole body glucose uptake determined by the glucose disposal rate during euglycemic clamp was acutely increased by 2.5 mg . kg(-1).min(-1) in response to ES and, moreover, remained elevated by 3 - 4 mg . kg(-1) . min(-1) for at least 90 min after cessation of stimulation. Thus the stimulatory effect of ES on whole body glucose uptake persisted not only during, but also after, stimulation. Low-frequency ES may become a useful therapeutic approach to activate energy and glucose metabolism in humans.