A large region controls tRNA gene transcription.
A large region controls tRNA gene transcription.
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一个大区域控制 tRNA 基因转录。
DOI：
10.1016/0022-2836(85)90209-8
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发表时间：
1985
影响因子：
5.6
通讯作者：
Sprague,KU
中科院分区：
文献类型：
--
作者：
Wilson,ET;Larson,D;Young,LS;Sprague,KU
我们发现tRNA基因转录受一个比之前描述的A和B盒内部片段大得多的区域控制。在家蚕tRNA2Alagene中，转录上重要的序列至少从−13延伸到+146，因此包括98碱基对初级转录单位的上游和下游序列。我们表明，这个控制区的外观大小可以通过用于测量体外转录的条件来操纵。具体地说，使用高浓度的模板来克服粗提物中抑制物质的影响可以使控制区看起来更小。我们认为，这一发现解释了观察到的不同tRNA基因转录序列要求的大部分差异。如果是这样的话，大尺寸很可能是tRNA基因控制区的一个普遍特征。
We find that tRNA gene transcription is controlled by a region much larger than the previously described internal segments known as A and B boxes. In aBombyx morisilkworm tRNA2Alagene, transcriptionally important sequences extend at least from −13 to + 146, and thus include sequences both upstream and downstream from the 98 base-pair primary transcription unit. We show that the apparent size of this control region can be manipulated by the conditions used to measure transcriptionin vitro. Specifically, the use of high concentrations of templates to overcome the effect of an inhibitory substance in crude extracts can make the control region appear smaller. We propose that this finding explains much of the observed variability in the sequence requirements for transcription of different tRNA genes. If so, large size is likely to be a general feature of tRNA gene control regions.