An instrument for simultaneous acquisition of fluorescence spectra and fluorescence lifetimes from single cells.
An instrument for simultaneous acquisition of fluorescence spectra and fluorescence lifetimes from single cells.
复制标题
一种同时采集单细胞荧光光谱和荧光寿命的仪器。
DOI：
10.1111/j.1751-1097.1987.tb08419.x
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发表时间：
1987
影响因子：
3.3
通讯作者：
Rodgers,MA
中科院分区：
文献类型：
--
作者：
Ramponi,R;Rodgers,MA
描述了一种仪器，该仪器具有获得分散在生物细胞中的荧光物种的光谱和寿命的能力。它在单细胞（或细胞器）水平上工作，光谱和时间数据收集可以同时进行。同步泵浦锁模染料激光器提供激发光，时间相关单光子计数用于寿命测量，二极管阵列光谱仪用于光谱工作。利用荧光显微镜获得亚微米的空间分辨率。时间分辨率优于300ps，波长分辨率小于1nm。该仪器已用于观察小鼠骨髓瘤细胞血卟啉的光谱和时间特征。
An instrument is described that has the capability of acquiring both the spectrum and lifetime(s) of fluorescent species dispersed in biological cells. It operates at the single cell (or organelle) level and the spectral and temporal data collection can be performed simultaneously. A synchronously pumped, mode‐locked dye laser provides the excitation light, time‐correlated single‐photon counting is used for lifetime measurements, and a diode array spectrograph is used for spectral work. Spatial resolution of sub‐micrometer is obtained using a fluorescence microscope. The temporal resolution is better than 300 ps and wavelength resolution is less than 1 nm per channel. The instrument has been used for observing the spectral and temporal characteristics of hematoporphyrin in mouse myeloma cells.