Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7
Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7
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DOI：
10.1016/j.carres.2012.08.019
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发表时间：
2012-11-01
影响因子：
3.1
通讯作者：
Shchyogolev, Sergei Yu.
中科院分区：
文献类型：
--
作者：
Belyakov, Alexei Ye.;Burygin, Gennady L.;Shchyogolev, Sergei Yu.
这是第一份在细菌鞭毛蛋白（鞭毛的一种结构蛋白）中鉴定出O-连接重复聚糖的报告。通过糖分析、Smith降解、H-1和C-13 NMR光谱和质谱的研究表明，促进植物生长的根细菌巴西固氮菌Sp 7的极性鞭毛鞭毛蛋白的聚糖链由分子量为7.7 kDa的多糖表示，其具有以下结构的支链四糖重复单元：-> 3）-alpha-L-Rhap-（1 -> 3）-beta-D-Galp-（1 -> 3）-beta-D-GlcpNAc-（1 -> 4 up arrow 1 alpha-L-Fucp（C）2012由Elsevier Ltd.
This is the first report to have identified an O-linked repetitive glycan in bacterial flagellin, a structural protein of the flagellum. Studies by sugar analysis, Smith degradation, H-1 and C-13 NMR spectroscopy, and mass spectrometry showed that the glycan chains of the polar flagellum flagellin of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 are represented by a polysaccharide with a molecular mass of 7.7 kDa, which has a branched tetrasaccharide repeating unit of the following structure: -> 3)-alpha-L-Rhap-(1 -> 3)-beta-D-Galp-(1 -> 3)-beta-D-GlcpNAc-(1 -> 4 up arrow 1 alpha-L-Fucp(C) 2012 Published by Elsevier Ltd.