Palmitate binding to serum albumin, measured by rate of dialysis.
Palmitate binding to serum albumin, measured by rate of dialysis.
复制标题
棕榈酸酯与血清白蛋白的结合，通过透析速率测量。
DOI：
10.1111/j.1432-1033.1988.tb14060.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
S. Andersen
中科院分区：
文献类型：
--
作者：
R. Brodersen;B. Honoré;S. Andersen
在两个相同的样品溶液之间使用乙酰纤维素膜进行透析实验;在一侧添加微量放射性标记的棕榈酸盐，并测量标记物的透析平衡速率。通过与标准实验中测量的速率进行比较，使用纯白蛋白溶液，我们获得了用于结合棕榈酸酯的储备白蛋白浓度，该储备白蛋白浓度先前被定义为与标记配体结合的纯标准白蛋白的浓度，该浓度与样品中的标记配体结合得一样紧密。开发了两种技术，一种用于1毫升样品体积，另一种用于25微升。在纯白蛋白溶液中测量的用于结合棕榈酸酯的储备白蛋白在添加棕榈酸酯和硬脂酸酯时同样减少，油酸酯略少，亚油酸酯更少，表明棕榈酸酯和硬脂酸酯竞争性结合，而与不饱和酸结合的相互作用不太明显。氯离子与棕榈酸盐竞争结合。33名男性成人血清样本中的储备白蛋白浓度为420 +/- 59 microM（平均值+/- SD），33名女性为351 +/- 50 microM。10名新生儿的脐带血清平均为172 +/- 56 μ M。新生儿血清中的储备白蛋白浓度低于正常白蛋白浓度，正常白蛋白浓度约为600 μ M。这似乎表明存在对棕榈酸酯具有较低亲和力的白蛋白物质。
Dialysis experiments were performed with an acetylcellulose membrane between two identical sample solutions; a trace amount of radiolabelled palmitate was added on one side and the rate of dialytic equilibration of the label was measured. By comparison with rates measured in standard experiments, using pure albumin solutions, we obtained the reserve albumin concentration for binding of palmitate, previously defined as the concentration of a pure standard albumin which binds the labelled ligand as tightly as it is bound in the sample. Two techniques were developed, one for 1-ml sample volumes, another for 25 microliter. Reserve albumin for binding of palmitate, measured in pure albumin solutions, decreased equally on addition of palmitate and stearate, slightly less with oleate and still less with linoleate, indicating that palmitate and stearate are bound competitively while interaction with binding of the unsaturated acids is less pronounced. Chloride ions compete with binding of palmitate. Reserve albumin concentration in serum samples from 33 male adults was 420 +/- 59 microM (mean +/- SD), and in 33 females, 351 +/- 50 microM. Umbilical cord sera from ten newborn infants averaged 172 +/- 56 microM. The reserve albumin concentrations in the sera of newborn infants are low compared with the normal albumin concentration, which is about 600 microM. This appears to indicate the presence of an albumin species with lower affinity for palmitate.
影响因子：
--
作者：
A. Spector
通讯作者：
A. Spector
确定配体结合的储备白蛋白当量，探测蛋白质的两种不同的结合功能。
影响因子：
2.9
作者：
Brodersen,R;Andersen,S;Jacobsen,C;Sønderskov,O;Ebbesen,F;Cashore,WJ;Larsen,S
通讯作者：
Larsen,S