Morphological abnormalities in the hippocampus of the weaver mutant mouse.
Morphological abnormalities in the hippocampus of the weaver mutant mouse.
复制标题
织布突变小鼠海马形态异常。
DOI：
10.1016/0006-8993(95)00974-u
复制
发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Abe,H
中科院分区：
文献类型：
--
作者：
Sekiguchi,M;Nowakowski,RS;Nagato,Y;Tanaka,O;Guo,H;Madoka,M;Abe,H
采用Nissl和Timm染色法对纯合子B6CBA织鼠（wu wu）与正常B6CBA同窝鼠（+/+）和C57BL/6J小鼠的海马层析进行比较。在nissl染色的制备中，正常窝鼠在海马CA3区表现出紧凑、规则的锥体细胞排列。相比之下，在纯合子weaver突变小鼠中，CA3区的锥体细胞层往往比正常的更厚，神经pil明显增加，这可以从层内无细胞间隙的存在中得到证明。此外，在海马的背腹侧，还发现了小的异位锥体细胞簇，有时锥体细胞层细分为2或3层。在Timm对正常小鼠海马的染色制备中，可以看到两束明显分离的轴突从海门处出现：一束在CA3区锥体细胞层之上（即锥体上苔藓纤维层，SPMFL），另一束在锥体细胞层之下（即锥体下苔藓纤维层，IPMFL）。相反，在一些纯合子编织小鼠中，苔藓纤维束的起源明显不同于正常；具体来说，苔藓纤维从锥体上和锥体下苔藓纤维层之间的区域以弥漫性的方式出现。在其他编织小鼠中，短的不连续束从锥体下苔藓纤维层发散出来，侵入加厚的锥体细胞层。此外，异位锥体细胞位于IPMFL下方的CA3区。在编织突变体的海马中观察到的形态变化可能是继发于更基本的遗传缺陷。
The lamination of the hippocampus in the homozygous B6CBA weaver mouse (wu wu ) was compared with that in normal B6CBA littermates (+/+) and C57BL/6J mice using Nissl and Timm's staining. In Nissl-stained preparations, the normal littermates exhibit a compact, regular arrangement of pyramidal cells in area CA3 of the hippocampus. In contrast, in homozygous weaver mutant mice, the pyramidal cell layer of area CA3 frequently appears to be thicker than normal with an apparent increase of neuropil, as evidenced by the presence of cell-free spaces within the layer. Also, small ectopic clusters of pyramidal cells and sometimes the subdivision of the pyramidal cell layer into 2 or 3 layers were found throughout the dorsoventral extent of the hippocampus. In Timm's stained preparations of the normal mouse hippocampus, two clearly separated bundles of axons were seen emerging from the hilus: one bundle running above the pyramidal cell layer of area CA3 (i.e., the suprapyramidal mossy fiber layer, SPMFL), and the second bundle running below the pyramidal cell layer (i.e., the infrapyramidal mossy fiber layer, IPMFL). In contrast, in some homozygous weaver mice, the origin of the mossy fiber bundles is clearly different from normal; specifically, mossy fibers emerge in a diffuse fashion from the area between suprapyramidal and infrapyramidal mossy fiber layers. In other weaver mice, short, discontinuous bundles diverge from the infrapyramidal mossy fiber layer and invade the thickened pyramidal cell layer. In addition, ectopic pyramidal cells are situated below the IPMFL in area CA3. The morphological changes observed in hippocampus of weaver mutants are likely to be secondary to a more basic genetic defect.