A comparison of the cell lines used in meningioma research
A comparison of the cell lines used in meningioma research
复制标题
DOI：
10.1016/j.surneu.2007.06.031
复制
发表时间：
2008-09-01
影响因子：
--
通讯作者：
Jensen, Randy L.
中科院分区：
文献类型：
--
作者：
Ragel, Brian T.;Couldwell, William T.;Jensen, Randy L.
背景：永生细胞系和移植到动物模型中的手术标本来源的细胞系用于脑膜瘤研究。我们解决2个批评的小鼠异种移植物侧腹肿瘤模型：为什么肿瘤诱导率来自手术标本低和不一致？侧腹肿瘤是脑膜瘤吗？方法：脑膜瘤细胞培养物进行Giemsa带核型分析和流式细胞术。用显微镜、免疫化学和超微结构分析了皮下注射诱导的小鼠侧腹肿瘤。Giemsa带研究发现脑膜瘤具有简单核型（
Background: Immortal cell lines and cell lines derived from operative specimens transplanted into animal models are used in meningioma research. We address 2 criticisms of the mouse xenograft flank tumor model: Why are tumor induction rates derived from operative specimens low and inconsistent? Are flank tumors meningiomas?Methods: Meningioma cell cultures were processed for Giemsa-band karyotyping and flow cytometry. Mouse flank tumors induced Subcutaneously were analyzed microscopically, immunohistochemically, and ultrastructurally. Giemsa-band studies identified meningiomas with simple karyotype (